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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional de Colombia
2019
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| Online Access: | https://www.redalyc.org/articulo.oa?id=77660294011 https://www.redalyc.org/journal/776/77660294011/ https://www.redalyc.org/journal/776/77660294011/html/ https://www.redalyc.org/journal/776/77660294011/77660294011.epub https://www.redalyc.org/journal/776/77660294011/movil |
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Table of Contents:
- Microalgae protoplasts isolation and fusion for biotechnology research Danilo Echeverri Juliana Romo Néstor Giraldo Lucía Atehortúa Biología cell wall microalgae protoplast fusion strain improvement Enzymatic hydrolysis Protoplasts are microbial or vegetable cells lacking a cell wall. These can be obtained from microalgae by an enzymatic hydrolysis process in the presence of an osmotic stabilizer. In general, protoplasts are experimentally useful in physiological, genetic and biochemical studies, so their acquisition and fusion will continue to be an active research area in modern biotechnology. The fusion of protoplasts in microalgae constitutes a tool for strain improvement because it allows both intra and interspecific genetic recombination, resulting in organisms with new or improved characteristics of industrial interest. In this review we briefly describe the methodology for obtaining protoplasts, as well as fusion methods and the main applications of microalgal platforms. 2019 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77660294011 https://www.redalyc.org/journal/776/77660294011/ https://www.redalyc.org/journal/776/77660294011/html/ https://www.redalyc.org/journal/776/77660294011/77660294011.epub https://www.redalyc.org/journal/776/77660294011/movil 10.15446/rev.colomb.biote.v21n1.80248 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.1 Vol.XXI