Morfogénesis in vitro de Toona ciliata a partir de raquis de hojas jóvenes con tidiazuron
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| Format: | Artículo científico |
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Universidad Nacional de Colombia
2005
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| _version_ | 1876456851500957696 |
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| author | Marcos Daquinta |
| author_facet | Marcos Daquinta |
| contents | Morfogénesis in vitro de Toona ciliata a partir de raquis de hojas jóvenes con tidiazuron Marcos Daquinta Romelio Rodríguez Yarianne Lezcano Mariela Cid Danilo Pina Biología callus Meliacea Himalayan Cedar growth regulators plant regeneration The Meliacea are of great importance in construction and furniture-making. Toona ciliata is a Meliaceaoriginally from the Himalayan region; it is known as Himalayan Cedar in Cuba. Natural regeneration occursin this specie by seed diffusion and grafting; such propagation is limited. The object of this paper was topromote callus formation and plant regeneration in Toona ciliata from in vitro propagation. Two to three yearold mature trees (Toona ciliata) were used. Rachis were taken from young branches from these plants. Theywere disinfected in 0.25% (w/v) mercuric chloride solution for 10 min followed by three rinses in autoclaveddistilled-water. They were then established in MS supplemented with 0-1 mg/L thidiazuron culture medium.Nodular calluses were obtained having good morphogenic characteristics. Shoots sprouted fromsix-month-old calluses in the dark and plant regeneration was done in the light. AIB shoots were rooted inMS medium supplement with 1 mg/L IBA. 2005 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77670202 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.VII |
| format | Artículo científico |
| id | redalyc_77670202 |
| institution | Redalyc |
| language | en |
| publishDate | 2005 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Morfogénesis in vitro de Toona ciliata a partir de raquis de hojas jóvenes con tidiazuron Marcos Daquinta Biología callus Meliacea Himalayan Cedar growth regulators plant regeneration Morfogénesis in vitro de Toona ciliata a partir de raquis de hojas jóvenes con tidiazuron Marcos Daquinta Romelio Rodríguez Yarianne Lezcano Mariela Cid Danilo Pina Biología callus Meliacea Himalayan Cedar growth regulators plant regeneration The Meliacea are of great importance in construction and furniture-making. Toona ciliata is a Meliaceaoriginally from the Himalayan region; it is known as Himalayan Cedar in Cuba. Natural regeneration occursin this specie by seed diffusion and grafting; such propagation is limited. The object of this paper was topromote callus formation and plant regeneration in Toona ciliata from in vitro propagation. Two to three yearold mature trees (Toona ciliata) were used. Rachis were taken from young branches from these plants. Theywere disinfected in 0.25% (w/v) mercuric chloride solution for 10 min followed by three rinses in autoclaveddistilled-water. They were then established in MS supplemented with 0-1 mg/L thidiazuron culture medium.Nodular calluses were obtained having good morphogenic characteristics. Shoots sprouted fromsix-month-old calluses in the dark and plant regeneration was done in the light. AIB shoots were rooted inMS medium supplement with 1 mg/L IBA. 2005 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77670202 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.VII |
| title | Morfogénesis in vitro de Toona ciliata a partir de raquis de hojas jóvenes con tidiazuron |
| topic | Biología callus Meliacea Himalayan Cedar growth regulators plant regeneration |
| url | https://www.redalyc.org/articulo.oa?id=77670202 |