In vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria's association
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2022
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| _version_ | 1876427420379119616 |
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| author | Sussy Saymara Perera García |
| author_facet | Sussy Saymara Perera García |
| contents | In vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria's association Sussy Saymara Perera García Kalyanne Fernández Suárez Eduardo José Pérez Ortega Yonaisy Mujica Pérez Reneé Pérez-Pérez Yakelin Rodríguez Yon Geert Haesaert Biología Medio SRM MSR medium Cultivo in vitro In vitro culture Micorriza arbuscular In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1 - 3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21 - 25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp. 2022 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77674944004 https://www.redalyc.org/journal/776/77674944004/ https://www.redalyc.org/journal/776/77674944004/html/ https://www.redalyc.org/journal/776/77674944004/77674944004.epub https://www.redalyc.org/journal/776/77674944004/movil 10.15446/rev.colomb.biote.v24n2.100841 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.XXIV |
| format | Artículo científico |
| id | redalyc_77674944004 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | In vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria's association Sussy Saymara Perera García Biología Medio SRM MSR medium Cultivo in vitro In vitro culture Micorriza arbuscular In vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria's association Sussy Saymara Perera García Kalyanne Fernández Suárez Eduardo José Pérez Ortega Yonaisy Mujica Pérez Reneé Pérez-Pérez Yakelin Rodríguez Yon Geert Haesaert Biología Medio SRM MSR medium Cultivo in vitro In vitro culture Micorriza arbuscular In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1 - 3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21 - 25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp. 2022 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77674944004 https://www.redalyc.org/journal/776/77674944004/ https://www.redalyc.org/journal/776/77674944004/html/ https://www.redalyc.org/journal/776/77674944004/77674944004.epub https://www.redalyc.org/journal/776/77674944004/movil 10.15446/rev.colomb.biote.v24n2.100841 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.XXIV |
| title | In vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria's association |
| topic | Biología Medio SRM MSR medium Cultivo in vitro In vitro culture Micorriza arbuscular |
| url | https://www.redalyc.org/articulo.oa?id=77674944004 https://www.redalyc.org/journal/776/77674944004/ https://www.redalyc.org/journal/776/77674944004/html/ https://www.redalyc.org/journal/776/77674944004/77674944004.epub https://www.redalyc.org/journal/776/77674944004/movil |