Molecular Characterization of Colombian Yam Germoplasm by "Selective Amplification of Microsatellite Polymorphic Loci" (SAMPL)
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2006
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| _version_ | 1876490364520497152 |
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| author | Silvia Bustamante R. |
| author_facet | Silvia Bustamante R. |
| contents | Molecular Characterization of Colombian Yam Germoplasm by "Selective Amplification of Microsatellite Polymorphic Loci" (SAMPL) Silvia Bustamante R. Gustavo Buitrago H. Biología DNA Dioscorea spp microsatellites In Colombia, yam (Dioscorea spp.) has been a basic and traditional crop for small-and medium-scale farmers. Ithas been an important source of family employment in the Colombian Atlantic Coast for the past severalcenturies. However, yam is the most fragile crop regarding market issues and disease susceptibility (e.g. toanthracnose and viruses). At present, the crop is under progressive recovery from a previous drastic reductionin area and production, and apparently allows high profits. Is imperative for the future of the crop, that the ruralfarmers are supplied with selected varieties, thereby increasing their sustainability and economic yield. However,such varieties are not available, but have to be bred. Breeding of yam in turn is difficult, and presupposesknowledge about the genetic diversity in the breeding germplasm, which is not known. For these reasons, wecharacterized the Colombian yam varieties used in the fields as well as wild relatives with a molecular markertechnique: SAMPL (Selective Amplification of Microsatellite Polymorphic Loci). Positions of 160 unequivocallyscorable bands (of which 56 were monomorphic, 35% of total, with an average of 2.8 polymorphic bands perassay) were transformed into a binary character matrix and analyzed. The resulting similarity matrix wastransformed into a dendrogram with the UPGMA algorithm. The diversity of the collections with this techniquewas 0.0471 with a mean similarity of 0.9529. 2006 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77680207 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.VIII |
| format | Artículo científico |
| id | redalyc_77680207 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Molecular Characterization of Colombian Yam Germoplasm by "Selective Amplification of Microsatellite Polymorphic Loci" (SAMPL) Silvia Bustamante R. Biología DNA Dioscorea spp microsatellites Molecular Characterization of Colombian Yam Germoplasm by "Selective Amplification of Microsatellite Polymorphic Loci" (SAMPL) Silvia Bustamante R. Gustavo Buitrago H. Biología DNA Dioscorea spp microsatellites In Colombia, yam (Dioscorea spp.) has been a basic and traditional crop for small-and medium-scale farmers. Ithas been an important source of family employment in the Colombian Atlantic Coast for the past severalcenturies. However, yam is the most fragile crop regarding market issues and disease susceptibility (e.g. toanthracnose and viruses). At present, the crop is under progressive recovery from a previous drastic reductionin area and production, and apparently allows high profits. Is imperative for the future of the crop, that the ruralfarmers are supplied with selected varieties, thereby increasing their sustainability and economic yield. However,such varieties are not available, but have to be bred. Breeding of yam in turn is difficult, and presupposesknowledge about the genetic diversity in the breeding germplasm, which is not known. For these reasons, wecharacterized the Colombian yam varieties used in the fields as well as wild relatives with a molecular markertechnique: SAMPL (Selective Amplification of Microsatellite Polymorphic Loci). Positions of 160 unequivocallyscorable bands (of which 56 were monomorphic, 35% of total, with an average of 2.8 polymorphic bands perassay) were transformed into a binary character matrix and analyzed. The resulting similarity matrix wastransformed into a dendrogram with the UPGMA algorithm. The diversity of the collections with this techniquewas 0.0471 with a mean similarity of 0.9529. 2006 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77680207 en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.2 Vol.VIII |
| title | Molecular Characterization of Colombian Yam Germoplasm by "Selective Amplification of Microsatellite Polymorphic Loci" (SAMPL) |
| topic | Biología DNA Dioscorea spp microsatellites |
| url | https://www.redalyc.org/articulo.oa?id=77680207 |