Human Papillomavirus Genotyping in Colombian Patients with Esophageal Squamous Cell Carcinoma and Gastric Carcinoma
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| Format: | Artículo científico |
| Language: | en |
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Universidad del Norte
2024
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| author | ISABEL ALMONACID URREGO |
| author_facet | ISABEL ALMONACID URREGO |
| contents | Human Papillomavirus Genotyping in Colombian Patients with Esophageal Squamous Cell Carcinoma and Gastric Carcinoma ISABEL ALMONACID URREGO CARMEN ALMONACID URREGO EDITH HERNÁNDEZ ROJAS SONIA ROSAS ARANGO Salud poly carcinoma Human papillomavirus merase chain reaction esophageal squamous cell carcinoma Introduction: Human papillomavirus (HPV) is one of the most important oncogenic viruses detected in different types of cancer, which includes esophageal squamous cell carcinoma (ESCC) and gastric cancer (GC). However, Colombia does not have data on the association of HPV with GC.Objetive: The aim of this study was to determine the presence HPV genotypes in patients with ESCC and GC, diagnosed between 2007 and 2014 at the Hospital Central de la Policía Nacional of Bogotá-Colombia (HOCEN).Methods: A total of 33 paraffin embedded tissue samples were examinate by polymerase chain reaction for the L1 gene of HPV. The extracted DNA was amplified using the universal primers MY09 and MY11. Then, the positive samples had high risk HPV genotyping performed on them. The samples were analyzed by Roche Cobas 4800 system.Results: Five (15%) samples identified as positive to HPV DNA four men (80%) and one woman (20%), one (20%) was positive for HPV-16. The other four, whose genotypes had not detected by COBAS 4800, samples could correspond to low-risk HPV, or match to some of the high-risk genotypes that had not included in the detection panel of this test. Also, four (80%) HPV DNA positives had identified as adenocarcinoma intestinal type, and one (20%) as ESCC.Conclusion: Our study proves the existance of HPV and p16 in Colombian patients with ESCC and GC, that could be a risk factor for the development of these pathologies in this population. 2024 artículo científico 0120-5552 https://www.redalyc.org/articulo.oa?id=81782334007 https://www.redalyc.org/journal/817/81782334007/ https://www.redalyc.org/journal/817/81782334007/html/ https://www.redalyc.org/journal/817/81782334007/81782334007.epub https://www.redalyc.org/journal/817/81782334007/movil 10.14482/sun.40.03.006.258 en http://www.redalyc.org/revista.oa?id=817 Salud Uninorte application/pdf Universidad del Norte Salud Uninorte (Colombia) Num.3 Vol.40 |
| format | Artículo científico |
| id | redalyc_81782334007 |
| institution | Redalyc |
| language | en |
| publishDate | 2024 |
| publisher | Universidad del Norte |
| spellingShingle | Human Papillomavirus Genotyping in Colombian Patients with Esophageal Squamous Cell Carcinoma and Gastric Carcinoma ISABEL ALMONACID URREGO Salud poly carcinoma Human papillomavirus merase chain reaction esophageal squamous cell carcinoma Human Papillomavirus Genotyping in Colombian Patients with Esophageal Squamous Cell Carcinoma and Gastric Carcinoma ISABEL ALMONACID URREGO CARMEN ALMONACID URREGO EDITH HERNÁNDEZ ROJAS SONIA ROSAS ARANGO Salud poly carcinoma Human papillomavirus merase chain reaction esophageal squamous cell carcinoma Introduction: Human papillomavirus (HPV) is one of the most important oncogenic viruses detected in different types of cancer, which includes esophageal squamous cell carcinoma (ESCC) and gastric cancer (GC). However, Colombia does not have data on the association of HPV with GC.Objetive: The aim of this study was to determine the presence HPV genotypes in patients with ESCC and GC, diagnosed between 2007 and 2014 at the Hospital Central de la Policía Nacional of Bogotá-Colombia (HOCEN).Methods: A total of 33 paraffin embedded tissue samples were examinate by polymerase chain reaction for the L1 gene of HPV. The extracted DNA was amplified using the universal primers MY09 and MY11. Then, the positive samples had high risk HPV genotyping performed on them. The samples were analyzed by Roche Cobas 4800 system.Results: Five (15%) samples identified as positive to HPV DNA four men (80%) and one woman (20%), one (20%) was positive for HPV-16. The other four, whose genotypes had not detected by COBAS 4800, samples could correspond to low-risk HPV, or match to some of the high-risk genotypes that had not included in the detection panel of this test. Also, four (80%) HPV DNA positives had identified as adenocarcinoma intestinal type, and one (20%) as ESCC.Conclusion: Our study proves the existance of HPV and p16 in Colombian patients with ESCC and GC, that could be a risk factor for the development of these pathologies in this population. 2024 artículo científico 0120-5552 https://www.redalyc.org/articulo.oa?id=81782334007 https://www.redalyc.org/journal/817/81782334007/ https://www.redalyc.org/journal/817/81782334007/html/ https://www.redalyc.org/journal/817/81782334007/81782334007.epub https://www.redalyc.org/journal/817/81782334007/movil 10.14482/sun.40.03.006.258 en http://www.redalyc.org/revista.oa?id=817 Salud Uninorte application/pdf Universidad del Norte Salud Uninorte (Colombia) Num.3 Vol.40 |
| title | Human Papillomavirus Genotyping in Colombian Patients with Esophageal Squamous Cell Carcinoma and Gastric Carcinoma |
| topic | Salud poly carcinoma Human papillomavirus merase chain reaction esophageal squamous cell carcinoma |
| url | https://www.redalyc.org/articulo.oa?id=81782334007 https://www.redalyc.org/journal/817/81782334007/ https://www.redalyc.org/journal/817/81782334007/html/ https://www.redalyc.org/journal/817/81782334007/81782334007.epub https://www.redalyc.org/journal/817/81782334007/movil |