Estandarización del método propuesto por la Organización Mundial de la Salud para determinar los niveles de susceptibilidad de los vectores de leishmaniasis a insecticidas
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Instituto Nacional de Salud
2002
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| _version_ | 1876490994458820608 |
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| author | Erika Santamaría |
| author_facet | Erika Santamaría |
| contents | Estandarización del método propuesto por la Organización Mundial de la Salud para determinar los niveles de susceptibilidad de los vectores de leishmaniasis a insecticidas Erika Santamaría Leonard E. Munstermann Medicina Lutzomyia bioassays insecticide susceptibility The WHO method for determining insecticide resistance was standardized for several speciesof Lutzomyia sand flies under laboratory and field conditions. The biological assays were appliedsolely to optimize the conditions for the control, i.e., without insecticide, and to estimate mortalitydue to handling or other unfavorable conditions. Adult female flies from 3 laboratory coloniesand one field strain were tested: two laboratory strains of Lutzomyia longipalpis, one laboratorystrain of Lutzomyia serrana and one field-collected strain of Lutzomyia quasitownsendi. TheWHO method was compared with one modified in which, during the post-exposure period, therecommended plain tube apparatus was replaced with a plastic container layered with dampplaster of Paris. Three paper substrate types were compared under each condition: olive oiladditive, silicon oil additive and plain paper. The measured variable was percent mortality in 24h. For the WHO protocol, the L. longipalpis strains indicated a 0-10% mortality, L. serrana 20-80% and L. quasitownsendi 10-50%. With the modified WHO apparatus, the average mortalitywas <4% for all species. No significant differences were observed among the paper treatments.These results indicate a strong species-specific effect of post-exposure conditions on sandflies. To establish baseline levels of insecticide resistance in Lutzomyia sand flies, the WHO method is recommended only for L. longipalpis, and the modified method for L. serrana, L.quasitownsendi and closely related species 2002 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84322215 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.22 |
| format | Artículo científico |
| id | redalyc_84322215 |
| institution | Redalyc |
| language | en |
| publishDate | 2002 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Estandarización del método propuesto por la Organización Mundial de la Salud para determinar los niveles de susceptibilidad de los vectores de leishmaniasis a insecticidas Erika Santamaría Medicina Lutzomyia bioassays insecticide susceptibility Estandarización del método propuesto por la Organización Mundial de la Salud para determinar los niveles de susceptibilidad de los vectores de leishmaniasis a insecticidas Erika Santamaría Leonard E. Munstermann Medicina Lutzomyia bioassays insecticide susceptibility The WHO method for determining insecticide resistance was standardized for several speciesof Lutzomyia sand flies under laboratory and field conditions. The biological assays were appliedsolely to optimize the conditions for the control, i.e., without insecticide, and to estimate mortalitydue to handling or other unfavorable conditions. Adult female flies from 3 laboratory coloniesand one field strain were tested: two laboratory strains of Lutzomyia longipalpis, one laboratorystrain of Lutzomyia serrana and one field-collected strain of Lutzomyia quasitownsendi. TheWHO method was compared with one modified in which, during the post-exposure period, therecommended plain tube apparatus was replaced with a plastic container layered with dampplaster of Paris. Three paper substrate types were compared under each condition: olive oiladditive, silicon oil additive and plain paper. The measured variable was percent mortality in 24h. For the WHO protocol, the L. longipalpis strains indicated a 0-10% mortality, L. serrana 20-80% and L. quasitownsendi 10-50%. With the modified WHO apparatus, the average mortalitywas <4% for all species. No significant differences were observed among the paper treatments.These results indicate a strong species-specific effect of post-exposure conditions on sandflies. To establish baseline levels of insecticide resistance in Lutzomyia sand flies, the WHO method is recommended only for L. longipalpis, and the modified method for L. serrana, L.quasitownsendi and closely related species 2002 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84322215 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.22 |
| title | Estandarización del método propuesto por la Organización Mundial de la Salud para determinar los niveles de susceptibilidad de los vectores de leishmaniasis a insecticidas |
| topic | Medicina Lutzomyia bioassays insecticide susceptibility |
| url | https://www.redalyc.org/articulo.oa?id=84322215 |