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| Autore principale: | |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Instituto Nacional de Salud
2003
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=84323213 |
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Sommario:
- Comparative effect of osmium tetroxide and ruthenium tetroxide on Penicillium sp. hyphae and Saccharomyces cerevisiae fungal cell wall ultrastructure Nelly Ordóñez Orlando Torres Fernández Medicina Penicillium sp fungal cell wall osmium tetroxide ruthenium tetroxide fungal ultrastructure The fungal cell wall viewed through the electron microscope appears transparent when fixedby the conventional osmium tetroxide method. However, ruthenium tetroxide post-fixing hasrevealed new details in the ultrastructure of Penicillium sp. hyphae and Saccharomycescerevisiae yeast. Most significant was the demonstration of two or three opaque diverse electrondense layers on the cell wall of each species. Two additional features were detected. Penicilliumsepta presented a three-layered appearance and budding S. cerevisiae yeast cell walls showedinner filiform cell wall protrusions into the cytoplasm. The combined use of osmium tetroxideand ruthenium tetroxide is recommended for post-fixing in electron microscopy studies of fungi 2003 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84323213 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.23