Expression of transforming growth factor-beta and platelet-derived growth factor in linear scleroderma
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Instituto Nacional de Salud
2003
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| _version_ | 1876486835142656000 |
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| author | José Félix Restrepo |
| author_facet | José Félix Restrepo |
| contents | Expression of transforming growth factor-beta and platelet-derived growth factor in linear scleroderma José Félix Restrepo Renato Guzmán Antonio Iglesias Gerzaín Rodríguez Medicina growth factors linear scleroderma Linear scleroderma (LS) is a localized form of scleroderma characterized by mononuclear cellinfiltration and fibroblast proliferation. In the later stages of the disease, excessive collagen isdeposited with concomitant skin and appendage atrophy. These symptoms suggest a breakdownof fibroblast cell function, and consequently, growth factors have been thought to play a role inthe pathogenesis of LS. The present study examined the expression of TGF-ß and PDGF in skinbiopsies obtained from patients with LS and from normal subjects. Samples were prepared forimmunohistochemistry. To identify TGF-ß, two polyclonal antibodies were used: TGF-ß1 (RaB4)and TGF-ß2 (CL-B1/29) and, to identify PDGF, two monoclonal antibodies were used: PDGF-AA(3E-205) and PDGF-BB (1F-133). Staining for TGF-ß1 and TGF-ß2 was observed around bloodvessels (endothelial cells), and sweat glands in both LS and normal skin. Staining for PDGF-AAand PDGF-BB was intense in endothelial cells and sweat glands in LS and normal skin.Mononuclear cell infiltrates and abnormal collagen bundles did not stain for TGF-ß or PDGF.The strength and extent of staining was evaluated in tissues using a scale from zero (nostaining) to four (strong staining). The amount of TGF-ß1, TGF-ß2, PDGF-AA and PDGF-BB wasfound similar in LS and normal skin. These results do not support the hypothesis that theexcessive fibroblast cell activity and abnormal collagen deposition observed in LS areassociated with downregulation of TGF-ß or PDGF. 2003 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84323406 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.23 |
| format | Artículo científico |
| id | redalyc_84323406 |
| institution | Redalyc |
| language | en |
| publishDate | 2003 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Expression of transforming growth factor-beta and platelet-derived growth factor in linear scleroderma José Félix Restrepo Medicina growth factors linear scleroderma Expression of transforming growth factor-beta and platelet-derived growth factor in linear scleroderma José Félix Restrepo Renato Guzmán Antonio Iglesias Gerzaín Rodríguez Medicina growth factors linear scleroderma Linear scleroderma (LS) is a localized form of scleroderma characterized by mononuclear cellinfiltration and fibroblast proliferation. In the later stages of the disease, excessive collagen isdeposited with concomitant skin and appendage atrophy. These symptoms suggest a breakdownof fibroblast cell function, and consequently, growth factors have been thought to play a role inthe pathogenesis of LS. The present study examined the expression of TGF-ß and PDGF in skinbiopsies obtained from patients with LS and from normal subjects. Samples were prepared forimmunohistochemistry. To identify TGF-ß, two polyclonal antibodies were used: TGF-ß1 (RaB4)and TGF-ß2 (CL-B1/29) and, to identify PDGF, two monoclonal antibodies were used: PDGF-AA(3E-205) and PDGF-BB (1F-133). Staining for TGF-ß1 and TGF-ß2 was observed around bloodvessels (endothelial cells), and sweat glands in both LS and normal skin. Staining for PDGF-AAand PDGF-BB was intense in endothelial cells and sweat glands in LS and normal skin.Mononuclear cell infiltrates and abnormal collagen bundles did not stain for TGF-ß or PDGF.The strength and extent of staining was evaluated in tissues using a scale from zero (nostaining) to four (strong staining). The amount of TGF-ß1, TGF-ß2, PDGF-AA and PDGF-BB wasfound similar in LS and normal skin. These results do not support the hypothesis that theexcessive fibroblast cell activity and abnormal collagen deposition observed in LS areassociated with downregulation of TGF-ß or PDGF. 2003 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84323406 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.23 |
| title | Expression of transforming growth factor-beta and platelet-derived growth factor in linear scleroderma |
| topic | Medicina growth factors linear scleroderma |
| url | https://www.redalyc.org/articulo.oa?id=84323406 |