Degeneration of primary afferent terminals following brachial plexus extensive avulsion injury in rats
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Instituto Nacional de Salud
2004
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| _version_ | 1876470493429628928 |
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| author | Manuel Nieto Sampedro |
| author_facet | Manuel Nieto Sampedro |
| contents | Degeneration of primary afferent terminals following brachial plexus extensive avulsion injury in rats Manuel Nieto Sampedro Vilma Muñetón Gómez Julian Scott Taylor Sharon Averill John V. Priestley Medicina calcitonin gene primary afferents cervical spinal cord dorsal root avulsion purinoreceptor (P2X3) Important breakthroughs in the understanding regeneration failure in an injured CNS havebeen made by studies of primary afferent neurons. Dorsal rhizotomy has provided anexperimental model of brachial plexus (BP) avulsion. This is an injury in which the centralbranches of primary afferents are disrupted at their point of entry into the spinal cord, bringingmotor and sensory dysfunction to the upper limbs. In the present work, the central axonalorganization of primary afferents was examined in control (without lesion) adult Wistar rats andin rats subjected to a C3-T3 rhizotomy. Specific sensory axon subtypes were recognized byapplication of antibodies to the calcitonin gene-related peptide (CGRP), the P2X3 purinoreceptor,the low-affinity p75-neurotrophin receptor and the retrograde tracer cholera toxin subunit β(TCβ). Other subtypes weres labeled with the lectin Griffonia simplicifolia IB4. Usingimmunohistochemistry and high resolution light microscopy, brachial plexus rhizotomy in adultrats has proven a reliable model for several neural deficits in humans. This lesion produceddifferent degrees of terminal degeneration in the several types of primary afferents whichdefine sub-populations of sensitive neurons. Between the C6 and C8 levels of the spinal cord,,deafferentation was partial for peptidergic GCRP-positive fibers, in contrast with elimination ofnon peptidergic and myelinated fibers. Dorsal rhizotomy has provided an adequate experimentalmodel to study sensory alterations such as acute pain and allodynia as well as factors thataffect regeneration into the CNS., Therefore, the differential deafferentation response must beconsidered inr the evaluation of therapies for nociception (pain) and regeneration for brachialplexus avulsion. The anatomical diffierences among the primary afferent subtypes also affecttheir roles in normal and damaged conditions 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324209 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.24 |
| format | Artículo científico |
| id | redalyc_84324209 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Degeneration of primary afferent terminals following brachial plexus extensive avulsion injury in rats Manuel Nieto Sampedro Medicina calcitonin gene primary afferents cervical spinal cord dorsal root avulsion purinoreceptor (P2X3) Degeneration of primary afferent terminals following brachial plexus extensive avulsion injury in rats Manuel Nieto Sampedro Vilma Muñetón Gómez Julian Scott Taylor Sharon Averill John V. Priestley Medicina calcitonin gene primary afferents cervical spinal cord dorsal root avulsion purinoreceptor (P2X3) Important breakthroughs in the understanding regeneration failure in an injured CNS havebeen made by studies of primary afferent neurons. Dorsal rhizotomy has provided anexperimental model of brachial plexus (BP) avulsion. This is an injury in which the centralbranches of primary afferents are disrupted at their point of entry into the spinal cord, bringingmotor and sensory dysfunction to the upper limbs. In the present work, the central axonalorganization of primary afferents was examined in control (without lesion) adult Wistar rats andin rats subjected to a C3-T3 rhizotomy. Specific sensory axon subtypes were recognized byapplication of antibodies to the calcitonin gene-related peptide (CGRP), the P2X3 purinoreceptor,the low-affinity p75-neurotrophin receptor and the retrograde tracer cholera toxin subunit β(TCβ). Other subtypes weres labeled with the lectin Griffonia simplicifolia IB4. Usingimmunohistochemistry and high resolution light microscopy, brachial plexus rhizotomy in adultrats has proven a reliable model for several neural deficits in humans. This lesion produceddifferent degrees of terminal degeneration in the several types of primary afferents whichdefine sub-populations of sensitive neurons. Between the C6 and C8 levels of the spinal cord,,deafferentation was partial for peptidergic GCRP-positive fibers, in contrast with elimination ofnon peptidergic and myelinated fibers. Dorsal rhizotomy has provided an adequate experimentalmodel to study sensory alterations such as acute pain and allodynia as well as factors thataffect regeneration into the CNS., Therefore, the differential deafferentation response must beconsidered inr the evaluation of therapies for nociception (pain) and regeneration for brachialplexus avulsion. The anatomical diffierences among the primary afferent subtypes also affecttheir roles in normal and damaged conditions 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324209 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.24 |
| title | Degeneration of primary afferent terminals following brachial plexus extensive avulsion injury in rats |
| topic | Medicina calcitonin gene primary afferents cervical spinal cord dorsal root avulsion purinoreceptor (P2X3) |
| url | https://www.redalyc.org/articulo.oa?id=84324209 |