Efectividad del tratamiento antihipertensivo en una muestra de pacientes colombianos
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| Format: | Artículo científico |
| Langue: | en |
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Instituto Nacional de Salud
2004
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| _version_ | 1876475874050572288 |
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| author | Juan Carlos Moncada |
| author_facet | Juan Carlos Moncada |
| contents | Efectividad del tratamiento antihipertensivo en una muestra de pacientes colombianos Juan Carlos Moncada Franciso Javier Osorio Giovanny Mesa Carlos A. Isaza Medicina COS p67phox phox cells NADPH oxidase polymorphisms The NADPH oxidase system plays a central role in the antimicrobial activity of phagocytes. Thissystem is initiated by the translocation of cytosolic proteins p67 phox, p47phox and p40phox to be inclose contact with membrane flavocytochrome b558. This event begins the electron transfer fromcytosolic NADPH to molecular oxygen to produce superoxide anions. Herein, a functionalanalysis is presented of p67 phox polymorphisms identified from healthy humans. Mutationswere generated in the p67phox cDNA by site-directed mutagenesis and then transiently expressedin COS7 cells that also expressed gp91phox, p22phox, and p47phox from stable transgenes. Thechanges Val166Ile, Pro329Ser and His389Gln correspond to possible polymorphisms identifiedin healthy individuals revealed a functional activity similar to COS phox cells transiently transfectedwith WT p67phox; therefore, these modifications are not associated with genetic deficiencies inNADPH oxidase. In conclusion, the COSphox system represents an easily transfectable modelfor analysis of NADPH oxidase function in intact cells. The analysis of mutant derivatives ofp67phox provides insight into molecular mechanisms by which this subunit regulates the NADPHoxidase 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324306 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.3 Vol.24 |
| format | Artículo científico |
| id | redalyc_84324306 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Efectividad del tratamiento antihipertensivo en una muestra de pacientes colombianos Juan Carlos Moncada Medicina COS p67phox phox cells NADPH oxidase polymorphisms Efectividad del tratamiento antihipertensivo en una muestra de pacientes colombianos Juan Carlos Moncada Franciso Javier Osorio Giovanny Mesa Carlos A. Isaza Medicina COS p67phox phox cells NADPH oxidase polymorphisms The NADPH oxidase system plays a central role in the antimicrobial activity of phagocytes. Thissystem is initiated by the translocation of cytosolic proteins p67 phox, p47phox and p40phox to be inclose contact with membrane flavocytochrome b558. This event begins the electron transfer fromcytosolic NADPH to molecular oxygen to produce superoxide anions. Herein, a functionalanalysis is presented of p67 phox polymorphisms identified from healthy humans. Mutationswere generated in the p67phox cDNA by site-directed mutagenesis and then transiently expressedin COS7 cells that also expressed gp91phox, p22phox, and p47phox from stable transgenes. Thechanges Val166Ile, Pro329Ser and His389Gln correspond to possible polymorphisms identifiedin healthy individuals revealed a functional activity similar to COS phox cells transiently transfectedwith WT p67phox; therefore, these modifications are not associated with genetic deficiencies inNADPH oxidase. In conclusion, the COSphox system represents an easily transfectable modelfor analysis of NADPH oxidase function in intact cells. The analysis of mutant derivatives ofp67phox provides insight into molecular mechanisms by which this subunit regulates the NADPHoxidase 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324306 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.3 Vol.24 |
| title | Efectividad del tratamiento antihipertensivo en una muestra de pacientes colombianos |
| topic | Medicina COS p67phox phox cells NADPH oxidase polymorphisms |
| url | https://www.redalyc.org/articulo.oa?id=84324306 |