Caracterización biológica y molecular del aislado CIBMUQ/HDC, una cepa colombiana de referencia para Toxoplasma gondii
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| Format: | Artículo científico |
| Language: | en |
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Instituto Nacional de Salud
2004
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| _version_ | 1876468802632286208 |
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| author | Jhon Carlos Castaño |
| author_facet | Jhon Carlos Castaño |
| contents | Caracterización biológica y molecular del aislado CIBMUQ/HDC, una cepa colombiana de referencia para Toxoplasma gondii Jhon Carlos Castaño Alejandra M. Giraldo Carolina Gallego Daniel Ajzenberg Ajzenberg Marie Laure Dardé Jorge Enrique Gómez Medicina strains virulence Toxoplasma congenital toxoplasmosis There are few reports about characterization strains of Toxoplasma gondii that analyze thedifferences between isolates from Europe or United States with those obtained in South America.The current study analyzes virulence data from the mouse model, the gene SAG2 polymorphismby PCR-RFLP and microsatellite analysis in a single Colombian isolate. The strain was isolatedfrom blood of a child with congenital toxoplasmosis, living in Armenia, Colombia. Analysis ofvirulence in the mouse showed that this strain has an LD100 of 10 tachyzoites. Both methods ofgenetic characterization demonstrated that this strain belonged to the clonal type 1 and wascalled HOM/CTCO/2002/CIBMUQ/BL/HDC (brief name: CIBMUQ/HDC). The CIBMUQ/HDCstrain is the first Colombian strain available as a reference strain for national and internationalresearchers 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324307 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.3 Vol.24 |
| format | Artículo científico |
| id | redalyc_84324307 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Caracterización biológica y molecular del aislado CIBMUQ/HDC, una cepa colombiana de referencia para Toxoplasma gondii Jhon Carlos Castaño Medicina strains virulence Toxoplasma congenital toxoplasmosis Caracterización biológica y molecular del aislado CIBMUQ/HDC, una cepa colombiana de referencia para Toxoplasma gondii Jhon Carlos Castaño Alejandra M. Giraldo Carolina Gallego Daniel Ajzenberg Ajzenberg Marie Laure Dardé Jorge Enrique Gómez Medicina strains virulence Toxoplasma congenital toxoplasmosis There are few reports about characterization strains of Toxoplasma gondii that analyze thedifferences between isolates from Europe or United States with those obtained in South America.The current study analyzes virulence data from the mouse model, the gene SAG2 polymorphismby PCR-RFLP and microsatellite analysis in a single Colombian isolate. The strain was isolatedfrom blood of a child with congenital toxoplasmosis, living in Armenia, Colombia. Analysis ofvirulence in the mouse showed that this strain has an LD100 of 10 tachyzoites. Both methods ofgenetic characterization demonstrated that this strain belonged to the clonal type 1 and wascalled HOM/CTCO/2002/CIBMUQ/BL/HDC (brief name: CIBMUQ/HDC). The CIBMUQ/HDCstrain is the first Colombian strain available as a reference strain for national and internationalresearchers 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324307 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.3 Vol.24 |
| title | Caracterización biológica y molecular del aislado CIBMUQ/HDC, una cepa colombiana de referencia para Toxoplasma gondii |
| topic | Medicina strains virulence Toxoplasma congenital toxoplasmosis |
| url | https://www.redalyc.org/articulo.oa?id=84324307 |