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| Format: | Artículo científico |
| Language: | en |
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Instituto Nacional de Salud
2004
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| Online Access: | https://www.redalyc.org/articulo.oa?id=84324409 |
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| _version_ | 1866815721576595456 |
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| author | Wilson Mejía |
| author_facet | Wilson Mejía |
| contents | Protein malnutrition up-regulates growth hormone receptor expression in rat splenic B lymphocytes Wilson Mejía Myriam Sánchez Medicina lymphocytes malnutrition growth hormone receptor The reciprocal interaction between the endocrine and immune systems has been the subject ofactive research during the last decade, and an important body of evidence has accumulatedsupporting the role of the GH/IGF axis in immune function. More recently, the GH/IGF axis hasbeen postulated as playing an important role in the modulation of stress conditions, such ascatabolic stages, aging-related disorders, immunodeficient aids patients and malnutrition.Whether these effects are exerted through endocrine, autocrine or paracrine mechanismsremains to be determined for different immune cell types and tissues. The aim of the currentstudy was to define which specific subsets of lymphocytes are the primary targets for GH action.In addition, the regulatory role of stress induced by protein restriction was investigated withrespect to the relative distribution of GH receptor positive lymphoid cells. Normal growing ratswere fed isocaloric diets with variable protein content (0, 4, 8, 12 and 20%) for a period of 14days. The lymphoid cells were then separated from spleen, lymph nodes and peripheral bloodlymphocytes. Flow cytometry analysis measured the binding characteristics of Fluos-rrGH tolymphocytes together with specific PE-labelled mAbs defining CD4+ and CD8+ T cells and Blymphocytes. The pattern of expression of the GH receptor differed among the lymphoid tissuesand cell subsets. Spleen was the most responsive organ to protein deprivation with highestGH receptor expression in B lymphocytes, followed by CD4+ T cells. As the protein intake wasdecreased from 20% to 0%, the percentage of GHR positive cells increased from 12% to 52%in splenic B lymphocytes and from 8% to 17% in CD4+ T cells. In contrast, only 10%-13% oflymphocytes in lymph nodes and 2%-4% in circulation, showed binding sites to GH associatedwith protein deprivation. In conclusion, the increase in GH receptors on lymphocytes undercatabolic stress induced by protein malnutrition gives support to the hypothesis of a modulatoryrole of the GH/IGF axis in preserving the homeostasis of immune tissues 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324409 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.24 |
| format | Artículo científico |
| id | redalyc_84324409 |
| language | en |
| publishDate | 2004 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Protein malnutrition up-regulates growth hormone receptor expression in rat splenic B lymphocytes Wilson Mejía Medicina lymphocytes malnutrition growth hormone receptor Protein malnutrition up-regulates growth hormone receptor expression in rat splenic B lymphocytes Wilson Mejía Myriam Sánchez Medicina lymphocytes malnutrition growth hormone receptor The reciprocal interaction between the endocrine and immune systems has been the subject ofactive research during the last decade, and an important body of evidence has accumulatedsupporting the role of the GH/IGF axis in immune function. More recently, the GH/IGF axis hasbeen postulated as playing an important role in the modulation of stress conditions, such ascatabolic stages, aging-related disorders, immunodeficient aids patients and malnutrition.Whether these effects are exerted through endocrine, autocrine or paracrine mechanismsremains to be determined for different immune cell types and tissues. The aim of the currentstudy was to define which specific subsets of lymphocytes are the primary targets for GH action.In addition, the regulatory role of stress induced by protein restriction was investigated withrespect to the relative distribution of GH receptor positive lymphoid cells. Normal growing ratswere fed isocaloric diets with variable protein content (0, 4, 8, 12 and 20%) for a period of 14days. The lymphoid cells were then separated from spleen, lymph nodes and peripheral bloodlymphocytes. Flow cytometry analysis measured the binding characteristics of Fluos-rrGH tolymphocytes together with specific PE-labelled mAbs defining CD4+ and CD8+ T cells and Blymphocytes. The pattern of expression of the GH receptor differed among the lymphoid tissuesand cell subsets. Spleen was the most responsive organ to protein deprivation with highestGH receptor expression in B lymphocytes, followed by CD4+ T cells. As the protein intake wasdecreased from 20% to 0%, the percentage of GHR positive cells increased from 12% to 52%in splenic B lymphocytes and from 8% to 17% in CD4+ T cells. In contrast, only 10%-13% oflymphocytes in lymph nodes and 2%-4% in circulation, showed binding sites to GH associatedwith protein deprivation. In conclusion, the increase in GH receptors on lymphocytes undercatabolic stress induced by protein malnutrition gives support to the hypothesis of a modulatoryrole of the GH/IGF axis in preserving the homeostasis of immune tissues 2004 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84324409 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.24 |
| title | Protein malnutrition up-regulates growth hormone receptor expression in rat splenic B lymphocytes |
| topic | Medicina lymphocytes malnutrition growth hormone receptor |
| url | https://www.redalyc.org/articulo.oa?id=84324409 |