Plasmodium falciparum: high frequency of pfcrt point mutations and emergence of new mutant haplotypes in Colombia
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| Format: | Artículo científico |
| Language: | en |
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Instituto Nacional de Salud
2008
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| _version_ | 1876472896649428992 |
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| author | Eliana Restrepo |
| author_facet | Eliana Restrepo |
| contents | Plasmodium falciparum: high frequency of pfcrt point mutations and emergence of new mutant haplotypes in Colombia Eliana Restrepo Jaime Carmona-Fonseca Amanda Maestre Medicina malaria mutation Colombia chloroquine antimalarials Introduction. Studies on the molecular epidemiology of antimalarial resistanceconstitute a useful tool to understand the events underlying treatment failure andresistance in falciparum malaria in Colombia. Several authors have reported on theefficacy of some molecular markers to predict drug resistance in Plasmodiumfalciparum and the P. falciparum pfcrt gene has been widely characterized in thiscontext.Objective. To study the frequency of pfcrt gene mutations in P. falciparum and theirassociation with treatment failure to the antimalarials chloroquine, mefloquine,amodiaquine and sulfadoxine/pyrimethamine in two highly malaria endemic regions,the Turbo and Bajo Cauca, regions of northwestern Colombia.Materials and methods. A representative sample of patients with non-complicatedfalciparum malaria was selected from each region for assessment of the treatmentresponse together with the status of codons 72, 74, 75 and 76 in the pfcrt gene usinga PCR-RFLP approach.Results. A high frequency of treatment failure to chloroquine (82%) and toamodiaquine (29%) was confirmed while mefloquine and combined therapy remainedeffective in clearing the infection. The presence of the T76 mutation in pfcrt wasconfirmed in all 172 samples; the most common haplotype was CMNT (67%).Conclusions. No significant association could be confirmed between a particularhaplotype and the treatment response in any of the treatment groups. Herein, wereport, for the first time in Colombia, presence of two haplotypes, SMET and SMNT;mixed mutant and wild-type alleles were found in 12% of the samples. 2008 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84328403 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.28 |
| format | Artículo científico |
| id | redalyc_84328403 |
| institution | Redalyc |
| language | en |
| publishDate | 2008 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Plasmodium falciparum: high frequency of pfcrt point mutations and emergence of new mutant haplotypes in Colombia Eliana Restrepo Medicina malaria mutation Colombia chloroquine antimalarials Plasmodium falciparum: high frequency of pfcrt point mutations and emergence of new mutant haplotypes in Colombia Eliana Restrepo Jaime Carmona-Fonseca Amanda Maestre Medicina malaria mutation Colombia chloroquine antimalarials Introduction. Studies on the molecular epidemiology of antimalarial resistanceconstitute a useful tool to understand the events underlying treatment failure andresistance in falciparum malaria in Colombia. Several authors have reported on theefficacy of some molecular markers to predict drug resistance in Plasmodiumfalciparum and the P. falciparum pfcrt gene has been widely characterized in thiscontext.Objective. To study the frequency of pfcrt gene mutations in P. falciparum and theirassociation with treatment failure to the antimalarials chloroquine, mefloquine,amodiaquine and sulfadoxine/pyrimethamine in two highly malaria endemic regions,the Turbo and Bajo Cauca, regions of northwestern Colombia.Materials and methods. A representative sample of patients with non-complicatedfalciparum malaria was selected from each region for assessment of the treatmentresponse together with the status of codons 72, 74, 75 and 76 in the pfcrt gene usinga PCR-RFLP approach.Results. A high frequency of treatment failure to chloroquine (82%) and toamodiaquine (29%) was confirmed while mefloquine and combined therapy remainedeffective in clearing the infection. The presence of the T76 mutation in pfcrt wasconfirmed in all 172 samples; the most common haplotype was CMNT (67%).Conclusions. No significant association could be confirmed between a particularhaplotype and the treatment response in any of the treatment groups. Herein, wereport, for the first time in Colombia, presence of two haplotypes, SMET and SMNT;mixed mutant and wild-type alleles were found in 12% of the samples. 2008 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84328403 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.28 |
| title | Plasmodium falciparum: high frequency of pfcrt point mutations and emergence of new mutant haplotypes in Colombia |
| topic | Medicina malaria mutation Colombia chloroquine antimalarials |
| url | https://www.redalyc.org/articulo.oa?id=84328403 |