Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites
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| Format: | Artículo científico |
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Instituto Nacional de Salud
2016
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| author | Elizabete de Jesus Inês |
| author_facet | Elizabete de Jesus Inês |
| contents | Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites Elizabete de Jesus Inês Flavia Thamiris Figueiredo Pacheco Milena Carneiro Pinto Patrícia Silva de Almeida Mendes Hugo da Costa-Ribeiro Jr Neci Matos Soares Márcia Cristina Aquino Teixeira Medicina child diagnosis flotation sedimentation Intestinal parasites Introduction: The diagnosis of intestinal parasitic infections depends on the parasite load, the specific gravity density of the parasite eggs, oocysts or cysts, and the density and viscosity of flotation or sedimentation medium where faeces are processed. Objective: To evaluate the concordance between zinc sulphate flotation and centrifugal sedimentation in the recovery of parasites in faecal samples of children. Materials and methods: Faecal samples of 330 children from day care centers were evaluated by zinc sulphate flotation and centrifugal sedimentation techniques. The frequencies of detection of parasites by each method were determined and the agreement between the diagnostic techniques was evaluated using the kappa index, with 95% confidence intervals. Results: The faecal flotation in zinc sulphate diagnosed significantly more cases of Trichuris trichiura infection when compared to centrifugal sedimentation (39/330; 11.8% vs. 13/330; 3.9%, p <0.001), with low diagnostic concordance between methods (kappa=0.264; 95% CI: 0.102-0.427). Moreover, all positive samples for Enterobius vermicularis eggs (n=5) and Strongyloides stercoralis larvae (n=3) were diagnosed only by zinc sulphate. No statistical differences were observed between methods for protozoa identification. Conclusions: The results showed that centrifugal flotation in zinc sulphate solution was significantly more likely to detect light helminths eggs such as those of T. trichiura and E. vermicularis in faeces than the centrifugal sedimentation process. 2016 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84348515005 https://www.redalyc.org/journal/843/84348515005/ https://www.redalyc.org/journal/843/84348515005/html/ https://www.redalyc.org/journal/843/84348515005/84348515005.epub https://www.redalyc.org/journal/843/84348515005/movil en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.36 |
| format | Artículo científico |
| id | redalyc_84348515005 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites Elizabete de Jesus Inês Medicina child diagnosis flotation sedimentation Intestinal parasites Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites Elizabete de Jesus Inês Flavia Thamiris Figueiredo Pacheco Milena Carneiro Pinto Patrícia Silva de Almeida Mendes Hugo da Costa-Ribeiro Jr Neci Matos Soares Márcia Cristina Aquino Teixeira Medicina child diagnosis flotation sedimentation Intestinal parasites Introduction: The diagnosis of intestinal parasitic infections depends on the parasite load, the specific gravity density of the parasite eggs, oocysts or cysts, and the density and viscosity of flotation or sedimentation medium where faeces are processed. Objective: To evaluate the concordance between zinc sulphate flotation and centrifugal sedimentation in the recovery of parasites in faecal samples of children. Materials and methods: Faecal samples of 330 children from day care centers were evaluated by zinc sulphate flotation and centrifugal sedimentation techniques. The frequencies of detection of parasites by each method were determined and the agreement between the diagnostic techniques was evaluated using the kappa index, with 95% confidence intervals. Results: The faecal flotation in zinc sulphate diagnosed significantly more cases of Trichuris trichiura infection when compared to centrifugal sedimentation (39/330; 11.8% vs. 13/330; 3.9%, p <0.001), with low diagnostic concordance between methods (kappa=0.264; 95% CI: 0.102-0.427). Moreover, all positive samples for Enterobius vermicularis eggs (n=5) and Strongyloides stercoralis larvae (n=3) were diagnosed only by zinc sulphate. No statistical differences were observed between methods for protozoa identification. Conclusions: The results showed that centrifugal flotation in zinc sulphate solution was significantly more likely to detect light helminths eggs such as those of T. trichiura and E. vermicularis in faeces than the centrifugal sedimentation process. 2016 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84348515005 https://www.redalyc.org/journal/843/84348515005/ https://www.redalyc.org/journal/843/84348515005/html/ https://www.redalyc.org/journal/843/84348515005/84348515005.epub https://www.redalyc.org/journal/843/84348515005/movil en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.4 Vol.36 |
| title | Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites |
| topic | Medicina child diagnosis flotation sedimentation Intestinal parasites |
| url | https://www.redalyc.org/articulo.oa?id=84348515005 https://www.redalyc.org/journal/843/84348515005/ https://www.redalyc.org/journal/843/84348515005/html/ https://www.redalyc.org/journal/843/84348515005/84348515005.epub https://www.redalyc.org/journal/843/84348515005/movil |