Phylogenetic signal at the Cytb - SertRNA -IG1- ND1 mitochondrial region in Anopheles ( Kerteszia ) neivai Howard, Dyar & Knab, 1913
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Instituto Nacional de Salud
2017
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| author | Andrés López-Rubio |
| author_facet | Andrés López-Rubio |
| contents | Phylogenetic signal at the Cytb - SertRNA -IG1- ND1 mitochondrial region in Anopheles ( Kerteszia ) neivai Howard, Dyar & Knab, 1913 Andrés López-Rubio Juan David Suaza Charles Porter Sandra Uribe Gabriel Bedoya Iván Darío Vélez Medicina Panamá malaria Colombia Anopheles Guatemala Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms.Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai.Materials and methods: Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene.Results: Thirty-six sequences for A. neivai and A. pholidotus were obtained.Conclusions: Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected. 2017 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84356911015 https://www.redalyc.org/journal/843/84356911015/ https://www.redalyc.org/journal/843/84356911015/html/ https://www.redalyc.org/journal/843/84356911015/84356911015.epub https://www.redalyc.org/journal/843/84356911015/movil 10.7705/biomedica.v34i2.3452 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.37 |
| format | Artículo científico |
| id | redalyc_84356911015 |
| institution | Redalyc |
| language | en |
| publishDate | 2017 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Phylogenetic signal at the Cytb - SertRNA -IG1- ND1 mitochondrial region in Anopheles ( Kerteszia ) neivai Howard, Dyar & Knab, 1913 Andrés López-Rubio Medicina Panamá malaria Colombia Anopheles Guatemala Phylogenetic signal at the Cytb - SertRNA -IG1- ND1 mitochondrial region in Anopheles ( Kerteszia ) neivai Howard, Dyar & Knab, 1913 Andrés López-Rubio Juan David Suaza Charles Porter Sandra Uribe Gabriel Bedoya Iván Darío Vélez Medicina Panamá malaria Colombia Anopheles Guatemala Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms.Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai.Materials and methods: Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene.Results: Thirty-six sequences for A. neivai and A. pholidotus were obtained.Conclusions: Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected. 2017 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84356911015 https://www.redalyc.org/journal/843/84356911015/ https://www.redalyc.org/journal/843/84356911015/html/ https://www.redalyc.org/journal/843/84356911015/84356911015.epub https://www.redalyc.org/journal/843/84356911015/movil 10.7705/biomedica.v34i2.3452 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.37 |
| title | Phylogenetic signal at the Cytb - SertRNA -IG1- ND1 mitochondrial region in Anopheles ( Kerteszia ) neivai Howard, Dyar & Knab, 1913 |
| topic | Medicina Panamá malaria Colombia Anopheles Guatemala |
| url | https://www.redalyc.org/articulo.oa?id=84356911015 https://www.redalyc.org/journal/843/84356911015/ https://www.redalyc.org/journal/843/84356911015/html/ https://www.redalyc.org/journal/843/84356911015/84356911015.epub https://www.redalyc.org/journal/843/84356911015/movil |