Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in a sampled Colombian population
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| Natura: | Artículo científico |
| Lingua: | en |
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Instituto Nacional de Salud
2019
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| _version_ | 1876453741899546624 |
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| author | Efrén Avendaño-Tamayo |
| author_facet | Efrén Avendaño-Tamayo |
| contents | Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in a sampled Colombian population Efrén Avendaño-Tamayo Alex Rúa María Victoria Parra-Marín Winston Rojas Omer Campo Juan Chacón-Duque Piedad Agudelo-Flórez Carlos F. Narváez Doris M. Salgado Bertha Nelly Restrepo Gabriel Bedoya Medicina toll Dengue genetic genetics Colombia Introduction: Host genetics is recognized as an influential factor for the development of dengue disease.Objective: This study evaluated the association of dengue with the polymorphisms rs8192284 for gene IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN.Materials and methods: Of the 292 surveyed subjects, 191 were confirmed for dengue fever and the remaining 101 were included as controls. The genotypes were resolved using polymerase chain reaction and restriction fragment length polymorphism (PCR- RFLP). In an attempt to determine the risk (Odds Ratio) of suffering dengue fever, data were analyzed using chi-square for alleles and logistic regression for both genotypes and allelic combinations. Confidence intervals were set to 95% for all tests regardless of the adjustment by either self-identification or ancestry.Results: For Afro-Colombians, the allele rs8192284 C offered protection against dengue OR=0.425,(0.204-0.887), p=0.020. The alleles rs7248637 A and rs3775290 A posed, respectively, an increased risk of dengue for Afro-Colombians OR=2.389, (1.170-4.879), p=0.015 and Mestizos OR=2.329, (1.283-4.226), p=0.005. The reproducibility for rs8192284 C/C OR=2.45, (1.05-5.76), p=0.013 remained after adjustment by Amerindian ancestry OR=2.52, (1.04-6.09), p=0.013. The reproducibility for rs3775290 A/A OR=2.48, (1.09-5.65), p=0.033 remained after adjustment by European OR=2.34, (1.02-5.35), p=0.048, Amerindian OR=2.49, (1.09-5.66), p=0.035, and African ancestry OR=2.37, (1.04-5.41), p=0.046. Finally, the association of dengue fever with the allelic combination CAG OR=2.07, (1.06-4.05), p=0.033 remained after adjustment by Amerindian ancestry OR=2.16, (1.09-4.28), p=0.028.Conclusions: Polymorphisms rs8192284 for IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN were associated with the susceptibility to suffer dengue fever in the sampled Colombian population. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84359527010 https://www.redalyc.org/journal/843/84359527010/ https://www.redalyc.org/journal/843/84359527010/html/ https://www.redalyc.org/journal/843/84359527010/84359527010.epub https://www.redalyc.org/journal/843/84359527010/movil 10.7705/biomedica.v39i1.4029 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.1 Vol.39 |
| format | Artículo científico |
| id | redalyc_84359527010 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in a sampled Colombian population Efrén Avendaño-Tamayo Medicina toll Dengue genetic genetics Colombia Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in a sampled Colombian population Efrén Avendaño-Tamayo Alex Rúa María Victoria Parra-Marín Winston Rojas Omer Campo Juan Chacón-Duque Piedad Agudelo-Flórez Carlos F. Narváez Doris M. Salgado Bertha Nelly Restrepo Gabriel Bedoya Medicina toll Dengue genetic genetics Colombia Introduction: Host genetics is recognized as an influential factor for the development of dengue disease.Objective: This study evaluated the association of dengue with the polymorphisms rs8192284 for gene IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN.Materials and methods: Of the 292 surveyed subjects, 191 were confirmed for dengue fever and the remaining 101 were included as controls. The genotypes were resolved using polymerase chain reaction and restriction fragment length polymorphism (PCR- RFLP). In an attempt to determine the risk (Odds Ratio) of suffering dengue fever, data were analyzed using chi-square for alleles and logistic regression for both genotypes and allelic combinations. Confidence intervals were set to 95% for all tests regardless of the adjustment by either self-identification or ancestry.Results: For Afro-Colombians, the allele rs8192284 C offered protection against dengue OR=0.425,(0.204-0.887), p=0.020. The alleles rs7248637 A and rs3775290 A posed, respectively, an increased risk of dengue for Afro-Colombians OR=2.389, (1.170-4.879), p=0.015 and Mestizos OR=2.329, (1.283-4.226), p=0.005. The reproducibility for rs8192284 C/C OR=2.45, (1.05-5.76), p=0.013 remained after adjustment by Amerindian ancestry OR=2.52, (1.04-6.09), p=0.013. The reproducibility for rs3775290 A/A OR=2.48, (1.09-5.65), p=0.033 remained after adjustment by European OR=2.34, (1.02-5.35), p=0.048, Amerindian OR=2.49, (1.09-5.66), p=0.035, and African ancestry OR=2.37, (1.04-5.41), p=0.046. Finally, the association of dengue fever with the allelic combination CAG OR=2.07, (1.06-4.05), p=0.033 remained after adjustment by Amerindian ancestry OR=2.16, (1.09-4.28), p=0.028.Conclusions: Polymorphisms rs8192284 for IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN were associated with the susceptibility to suffer dengue fever in the sampled Colombian population. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84359527010 https://www.redalyc.org/journal/843/84359527010/ https://www.redalyc.org/journal/843/84359527010/html/ https://www.redalyc.org/journal/843/84359527010/84359527010.epub https://www.redalyc.org/journal/843/84359527010/movil 10.7705/biomedica.v39i1.4029 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.1 Vol.39 |
| title | Evaluation of variants in IL6R, TLR3, and DC-SIGN genes associated with dengue in a sampled Colombian population |
| topic | Medicina toll Dengue genetic genetics Colombia |
| url | https://www.redalyc.org/articulo.oa?id=84359527010 https://www.redalyc.org/journal/843/84359527010/ https://www.redalyc.org/journal/843/84359527010/html/ https://www.redalyc.org/journal/843/84359527010/84359527010.epub https://www.redalyc.org/journal/843/84359527010/movil |