Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Instituto Nacional de Salud
2019
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| author | Militza Guzmán |
| author_facet | Militza Guzmán |
| contents | Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato Medicina beta bacterial community lactamases rug resistance Introduction: The treatment of urinary tract infections has become more challenging due to the increasing frequency of multidrug-resistant Escherichia coli in human populations. Objective: To characterize multidrug-resistant E. coli isolates causing community-acquired urinary tract infections in Cumaná, Venezuela, and associate possible risk factors for infection by extended-spectrum beta-lactamases (ESBL)-producing isolates. Materials and methods: We included all the patients with urinary tract infections attending the urology outpatient consultation and emergency unit in the Hospital de Cumaná, Estado Sucre, Venezuela, from January through June, 2014. blaTEM, blaSHV and blaCTX-M genes detection was carried out by PCR. Results: We found a high prevalence of multidrug-resistant E. coli (25.2%) with 20.4% of the isolates producing ESBL. The ESBL-producing isolates showed a high frequency (66.7%) of simultaneous resistance to trimethoprim-sulphamethoxazole, fluoroquinolones and aminoglycosides compared to non-producing isolates (2.4%). Of the resistant isolates, 65.4% carried the blaTEM gene, 34.6% the blaCTX-M and 23.1% the blaSHV. The blaCTX-M genes detected belonged to the CTX-M-1 and CTX-M-2 groups. Plasmid transfer was demonstrated by in vitro conjugation in 17 of the 26 ESBL-producing isolates. All three genes detected were transferred to the transconjugants. Age over 60 years, complicated urinary tract infections and previous use of a catheter predisposed patients to infection by ESBL-producing E. coli. Conclusions: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84359814009 https://www.redalyc.org/journal/843/84359814009/ https://www.redalyc.org/journal/843/84359814009/html/ https://www.redalyc.org/journal/843/84359814009/84359814009.epub https://www.redalyc.org/journal/843/84359814009/movil 10.7705/biomedica.v39i2.4030 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.1 Vol.39 |
| format | Artículo científico |
| id | redalyc_84359814009 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela Militza Guzmán Medicina beta bacterial community lactamases rug resistance Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato Medicina beta bacterial community lactamases rug resistance Introduction: The treatment of urinary tract infections has become more challenging due to the increasing frequency of multidrug-resistant Escherichia coli in human populations. Objective: To characterize multidrug-resistant E. coli isolates causing community-acquired urinary tract infections in Cumaná, Venezuela, and associate possible risk factors for infection by extended-spectrum beta-lactamases (ESBL)-producing isolates. Materials and methods: We included all the patients with urinary tract infections attending the urology outpatient consultation and emergency unit in the Hospital de Cumaná, Estado Sucre, Venezuela, from January through June, 2014. blaTEM, blaSHV and blaCTX-M genes detection was carried out by PCR. Results: We found a high prevalence of multidrug-resistant E. coli (25.2%) with 20.4% of the isolates producing ESBL. The ESBL-producing isolates showed a high frequency (66.7%) of simultaneous resistance to trimethoprim-sulphamethoxazole, fluoroquinolones and aminoglycosides compared to non-producing isolates (2.4%). Of the resistant isolates, 65.4% carried the blaTEM gene, 34.6% the blaCTX-M and 23.1% the blaSHV. The blaCTX-M genes detected belonged to the CTX-M-1 and CTX-M-2 groups. Plasmid transfer was demonstrated by in vitro conjugation in 17 of the 26 ESBL-producing isolates. All three genes detected were transferred to the transconjugants. Age over 60 years, complicated urinary tract infections and previous use of a catheter predisposed patients to infection by ESBL-producing E. coli. Conclusions: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84359814009 https://www.redalyc.org/journal/843/84359814009/ https://www.redalyc.org/journal/843/84359814009/html/ https://www.redalyc.org/journal/843/84359814009/84359814009.epub https://www.redalyc.org/journal/843/84359814009/movil 10.7705/biomedica.v39i2.4030 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.1 Vol.39 |
| title | Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
| topic | Medicina beta bacterial community lactamases rug resistance |
| url | https://www.redalyc.org/articulo.oa?id=84359814009 https://www.redalyc.org/journal/843/84359814009/ https://www.redalyc.org/journal/843/84359814009/html/ https://www.redalyc.org/journal/843/84359814009/84359814009.epub https://www.redalyc.org/journal/843/84359814009/movil |