Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community
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| Natura: | Artículo científico |
| Lingua: | en |
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Instituto Nacional de Salud
2019
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| _version_ | 1876451013159813120 |
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| author | Cielo León |
| author_facet | Cielo León |
| contents | Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González Medicina Rhodnius Reduviidae Triatominae Panstrongylus Chagas disease Introduction: From 2011 to 2016, 24 cases of Chagas disease were reported in Córdoba according to the national public health surveillance system (Sistema Nacional de Vigilancia en Salud Pública, Sivigila), but the information regarding Trypanosoma cruzi circulating strains and infection rates are unknown. Objectives: To establish the triatomine species with which people come in contact and recognize as Chagas disease vectors, as well as to assess the infection with trypanosomes and make an exploratory approach to host feeding preferences with the participation of the local community. Materials and methods: Triatomines sampling was conducted in 12 municipalities between 2011 and 2016; T. cruzi infection was established by k-PCR, SAT-PCR, while strain genotyping was done by mini-exon and SL-IR (spliced-leader intergenic region) sequence characterization. We also screened for blood sources. Results: Local community members collected the majority of triatomines and we identified three species: Rhodnius pallescens, Panstrongylus geniculatus, and Eratyrus cuspidatus. The overall T. cruzi infection rate in collected triatomines was 66.6% and we detected the TcIDOM and TcI sylvatic strains. Community-based insect collection allowed reporting the presence of P. geniculatus in two new disperse rural settlements, T. cruzi infection of P. geniculatus in Córdoba, and the first report of triatomines infected with T. cruzi in Montería municipality. Conclusions: These results revealed the presence of triatomines infected with T. cruzi inside dwellings in five municipalities of Córdoba. The dominant circulating T. cruzi strain was TcIDOM, a genotype associated with human Chagas disease and cardiomyopathies in Colombia. Our results highlight the importance of local community participation in entomological surveillance tasks. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84360152005 https://www.redalyc.org/journal/843/84360152005/ https://www.redalyc.org/journal/843/84360152005/html/ https://www.redalyc.org/journal/843/84360152005/84360152005.epub https://www.redalyc.org/journal/843/84360152005/movil 10.7705/biomedica.v39i2.3973 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.39 |
| format | Artículo científico |
| id | redalyc_84360152005 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community Cielo León Medicina Rhodnius Reduviidae Triatominae Panstrongylus Chagas disease Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community Cielo León Mario Iván Ortiz Catalina Tovar Jorge Negrete Ernesto Arroyo Camila González Medicina Rhodnius Reduviidae Triatominae Panstrongylus Chagas disease Introduction: From 2011 to 2016, 24 cases of Chagas disease were reported in Córdoba according to the national public health surveillance system (Sistema Nacional de Vigilancia en Salud Pública, Sivigila), but the information regarding Trypanosoma cruzi circulating strains and infection rates are unknown. Objectives: To establish the triatomine species with which people come in contact and recognize as Chagas disease vectors, as well as to assess the infection with trypanosomes and make an exploratory approach to host feeding preferences with the participation of the local community. Materials and methods: Triatomines sampling was conducted in 12 municipalities between 2011 and 2016; T. cruzi infection was established by k-PCR, SAT-PCR, while strain genotyping was done by mini-exon and SL-IR (spliced-leader intergenic region) sequence characterization. We also screened for blood sources. Results: Local community members collected the majority of triatomines and we identified three species: Rhodnius pallescens, Panstrongylus geniculatus, and Eratyrus cuspidatus. The overall T. cruzi infection rate in collected triatomines was 66.6% and we detected the TcIDOM and TcI sylvatic strains. Community-based insect collection allowed reporting the presence of P. geniculatus in two new disperse rural settlements, T. cruzi infection of P. geniculatus in Córdoba, and the first report of triatomines infected with T. cruzi in Montería municipality. Conclusions: These results revealed the presence of triatomines infected with T. cruzi inside dwellings in five municipalities of Córdoba. The dominant circulating T. cruzi strain was TcIDOM, a genotype associated with human Chagas disease and cardiomyopathies in Colombia. Our results highlight the importance of local community participation in entomological surveillance tasks. 2019 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84360152005 https://www.redalyc.org/journal/843/84360152005/ https://www.redalyc.org/journal/843/84360152005/html/ https://www.redalyc.org/journal/843/84360152005/84360152005.epub https://www.redalyc.org/journal/843/84360152005/movil 10.7705/biomedica.v39i2.3973 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.39 |
| title | Detection of Trypanosoma cruzi strains circulating in Córdoba department (Colombia) isolated from triatomines (Hemiptera: Reduviidae) collected by the community |
| topic | Medicina Rhodnius Reduviidae Triatominae Panstrongylus Chagas disease |
| url | https://www.redalyc.org/articulo.oa?id=84360152005 https://www.redalyc.org/journal/843/84360152005/ https://www.redalyc.org/journal/843/84360152005/html/ https://www.redalyc.org/journal/843/84360152005/84360152005.epub https://www.redalyc.org/journal/843/84360152005/movil |