Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14
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| Format: | Artículo científico |
| Sprache: | en |
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Instituto Nacional de Salud
2020
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| author | Rafael A. Guerrero |
| author_facet | Rafael A. Guerrero |
| contents | Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14 Rafael A. Guerrero Carlos A. Guerrero Fanny Guzmán Orlando Acosta Medicina therapy neoplasms Oncolytic viruses rotavirus infections Introduction: Cancer is the second leading cause of death in the United States, surpassed only by cardiovascular disease. However, cancer has now overtaken cardiovascular disease as the main cause of death in 12 countries in Western Europe. The burden of cancer is posing a major challenge to health care systems worldwide and demanding improvements in methods for cancer prevention, diagnosis, and treatment. Alternative and complementary strategies for orthodox surgery, radiotherapy, and chemotherapy need to be developed.Objective: To determine the oncolytic potential of tumor cell-adapted rotavirus in terms of their ability to infect and lysate murine myeloma Sp2/0-Ag14 cells.Materials and methods: We inoculated rotaviruses Wt1-5, WWM, TRUYO, ECwt-O, and WTEW in Sp2/0-Ag14 cells and we examined their infectious effects by immunocytochemistry, immunofluorescence, flow cytometry, and DNA fragmentation assays. Results: Rotavirus infection involved the participation of some heat shock proteins, of protein disulfide isomerase (PDI), and integrin β3. We detected the accumulation of viral antigens within the virus-inoculated cells and in the culture medium in all the rotavirus isolates examined. The rotavirus-induced cell death mechanism in Sp2/0-Ag14 cells involved changes in cell membrane permeability, chromatin condensation, and DNA fragmentation, which were compatible with cytotoxicity and apoptosis.Conclusions: The ability of the rotavirus isolates Wt1-5, WWM, TRUYO, ECwt-O, and WTEW to infect and cause cell death of Sp2/0-Ag14 cells through mechanisms that are compatible with virus-induced apoptosis makes them potential candidates as oncolytic agents. 2020 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84364615014 https://www.redalyc.org/journal/843/84364615014/ https://www.redalyc.org/journal/843/84364615014/html/ https://www.redalyc.org/journal/843/84364615014/84364615014.epub https://www.redalyc.org/journal/843/84364615014/movil 10.7705/biomedica.4916 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.40 |
| format | Artículo científico |
| id | redalyc_84364615014 |
| institution | Redalyc |
| language | en |
| publishDate | 2020 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14 Rafael A. Guerrero Medicina therapy neoplasms Oncolytic viruses rotavirus infections Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14 Rafael A. Guerrero Carlos A. Guerrero Fanny Guzmán Orlando Acosta Medicina therapy neoplasms Oncolytic viruses rotavirus infections Introduction: Cancer is the second leading cause of death in the United States, surpassed only by cardiovascular disease. However, cancer has now overtaken cardiovascular disease as the main cause of death in 12 countries in Western Europe. The burden of cancer is posing a major challenge to health care systems worldwide and demanding improvements in methods for cancer prevention, diagnosis, and treatment. Alternative and complementary strategies for orthodox surgery, radiotherapy, and chemotherapy need to be developed.Objective: To determine the oncolytic potential of tumor cell-adapted rotavirus in terms of their ability to infect and lysate murine myeloma Sp2/0-Ag14 cells.Materials and methods: We inoculated rotaviruses Wt1-5, WWM, TRUYO, ECwt-O, and WTEW in Sp2/0-Ag14 cells and we examined their infectious effects by immunocytochemistry, immunofluorescence, flow cytometry, and DNA fragmentation assays. Results: Rotavirus infection involved the participation of some heat shock proteins, of protein disulfide isomerase (PDI), and integrin β3. We detected the accumulation of viral antigens within the virus-inoculated cells and in the culture medium in all the rotavirus isolates examined. The rotavirus-induced cell death mechanism in Sp2/0-Ag14 cells involved changes in cell membrane permeability, chromatin condensation, and DNA fragmentation, which were compatible with cytotoxicity and apoptosis.Conclusions: The ability of the rotavirus isolates Wt1-5, WWM, TRUYO, ECwt-O, and WTEW to infect and cause cell death of Sp2/0-Ag14 cells through mechanisms that are compatible with virus-induced apoptosis makes them potential candidates as oncolytic agents. 2020 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84364615014 https://www.redalyc.org/journal/843/84364615014/ https://www.redalyc.org/journal/843/84364615014/html/ https://www.redalyc.org/journal/843/84364615014/84364615014.epub https://www.redalyc.org/journal/843/84364615014/movil 10.7705/biomedica.4916 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.40 |
| title | Assessing the oncolytic potential of rotavirus on mouse myeloma cell line Sp2/0-Ag14 |
| topic | Medicina therapy neoplasms Oncolytic viruses rotavirus infections |
| url | https://www.redalyc.org/articulo.oa?id=84364615014 https://www.redalyc.org/journal/843/84364615014/ https://www.redalyc.org/journal/843/84364615014/html/ https://www.redalyc.org/journal/843/84364615014/84364615014.epub https://www.redalyc.org/journal/843/84364615014/movil |