Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats
Fuente:
Redalyc
Enregistré dans:
| Auteur principal: | |
|---|---|
| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Instituto Nacional de Salud
2023
|
| Sujets: | |
| Accès en ligne: | |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1876422775934025728 |
|---|---|
| author | Aline Garnevi-Fávero |
| author_facet | Aline Garnevi-Fávero |
| contents | Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats Aline Garnevi-Fávero Karina Nascimento-da Silva Willian Rodrigues-Ribeiro Caroline Marcantonio-Ferreira Patrícia Sartorelli Leonardo Cardili Rita de Cássia-Sinigaglia Joice Naiara Bertaglia-Pereira Marcelo Aparecido-da Silva Wagner Vilegas Marcelo José Dias-Silva Ana Paula Ribeiro-Paiotti Medicina Mimosa colitis ulcerative herbal medicine inflammatory bowel diseases Introduction. Anti-inflammatories, immunosuppressants, and immunobiological are commonly used in the treatment of inflammatory bowel disease. However, some patients do not present an adequate response or lose effective response during the treatment. A recent study found a potential anti-inflammatory effect of the hydroalcoholic extract of Mimosa caesalpiniifolia on trinitrobenzene sulfonic acid-induced colitis in Wistar rats.Objective. To evaluate the effects of M. caesalpiniifolia pre-formulation on the intestinal barrier using dextran sulfate sodium-induced colitis model.Materials and methods. Leaf extracts were prepared in 70% ethanol and dried with a Buchi B19 Mini-spray dryer using 20% Aerosil® solution. Thirty-two male Wistar rats were randomized into four groups: basal control, untreated colitis, pre-formulation control (125 mg/kg/day), and colitis treated with pre-formulation (125 mg/kg/day). Clinical activity index was recorded daily and all rats were euthanized on the ninth day. Colon fragments were fixed and processed for histological and ultrastructural analyses. Stool samples were collected and processed for analysis of the short-chain fatty acid.Results. Treatment with the pre-formulation decreased the clinical activity (bloody diarrhea), inflammatory infiltrate, and the ulcers. Pre-formulation did not repair the epithelial barrier and there were no significant differences in the goblet cells index. There was a significant difference in butyrate levels in the rats treated with the pre-formulation. Conclusions. The pre-formulation minimized the clinical symptoms of colitis and intestinal inflammation, but did not minimize damage to the intestinal barrier. 2023 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84375798013 https://www.redalyc.org/journal/843/84375798013/ https://www.redalyc.org/journal/843/84375798013/html/ https://www.redalyc.org/journal/843/84375798013/84375798013.epub https://www.redalyc.org/journal/843/84375798013/movil https://doi.org/10.7705/biomedica.6611 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.43 |
| format | Artículo científico |
| id | redalyc_84375798013 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Instituto Nacional de Salud |
| spellingShingle | Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats Aline Garnevi-Fávero Medicina Mimosa colitis ulcerative herbal medicine inflammatory bowel diseases Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats Aline Garnevi-Fávero Karina Nascimento-da Silva Willian Rodrigues-Ribeiro Caroline Marcantonio-Ferreira Patrícia Sartorelli Leonardo Cardili Rita de Cássia-Sinigaglia Joice Naiara Bertaglia-Pereira Marcelo Aparecido-da Silva Wagner Vilegas Marcelo José Dias-Silva Ana Paula Ribeiro-Paiotti Medicina Mimosa colitis ulcerative herbal medicine inflammatory bowel diseases Introduction. Anti-inflammatories, immunosuppressants, and immunobiological are commonly used in the treatment of inflammatory bowel disease. However, some patients do not present an adequate response or lose effective response during the treatment. A recent study found a potential anti-inflammatory effect of the hydroalcoholic extract of Mimosa caesalpiniifolia on trinitrobenzene sulfonic acid-induced colitis in Wistar rats.Objective. To evaluate the effects of M. caesalpiniifolia pre-formulation on the intestinal barrier using dextran sulfate sodium-induced colitis model.Materials and methods. Leaf extracts were prepared in 70% ethanol and dried with a Buchi B19 Mini-spray dryer using 20% Aerosil® solution. Thirty-two male Wistar rats were randomized into four groups: basal control, untreated colitis, pre-formulation control (125 mg/kg/day), and colitis treated with pre-formulation (125 mg/kg/day). Clinical activity index was recorded daily and all rats were euthanized on the ninth day. Colon fragments were fixed and processed for histological and ultrastructural analyses. Stool samples were collected and processed for analysis of the short-chain fatty acid.Results. Treatment with the pre-formulation decreased the clinical activity (bloody diarrhea), inflammatory infiltrate, and the ulcers. Pre-formulation did not repair the epithelial barrier and there were no significant differences in the goblet cells index. There was a significant difference in butyrate levels in the rats treated with the pre-formulation. Conclusions. The pre-formulation minimized the clinical symptoms of colitis and intestinal inflammation, but did not minimize damage to the intestinal barrier. 2023 artículo científico 0120-4157 https://www.redalyc.org/articulo.oa?id=84375798013 https://www.redalyc.org/journal/843/84375798013/ https://www.redalyc.org/journal/843/84375798013/html/ https://www.redalyc.org/journal/843/84375798013/84375798013.epub https://www.redalyc.org/journal/843/84375798013/movil https://doi.org/10.7705/biomedica.6611 en http://www.redalyc.org/revista.oa?id=843 Biomédica application/pdf Instituto Nacional de Salud Biomédica (Colombia) Num.2 Vol.43 |
| title | Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar rats |
| topic | Medicina Mimosa colitis ulcerative herbal medicine inflammatory bowel diseases |
| url | https://www.redalyc.org/articulo.oa?id=84375798013 https://www.redalyc.org/journal/843/84375798013/ https://www.redalyc.org/journal/843/84375798013/html/ https://www.redalyc.org/journal/843/84375798013/84375798013.epub https://www.redalyc.org/journal/843/84375798013/movil https://doi.org/10.7705/biomedica.6611 |