UV-Vis spectroscopy and chemometric: a simple method to differentiate honey, partially ripe honey and nectars
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| Format: | Artículo científico |
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Instituto Nacional de Tecnología Agropecuaria
2023
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| _version_ | 1876467921855709184 |
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| author | L. M. Maldonado |
| author_facet | L. M. Maldonado |
| contents | UV-Vis spectroscopy and chemometric: a simple method to differentiate honey, partially ripe honey and nectars L. M. Maldonado K. Marcinkevicius A.R. Álvarez V.M. Salomón M. Gaggiotti N. Messina M.A. Palacio S. Medina M.M. Maldonado E. Orellano E. L. Bedascarrasbure Agrociencias UV Honey nectar chemometrics Vis spectroscopy Honey is a supersaturated solution of sugars that also contains small amounts of enzymes, vitamins, phenolic compounds (e.g., flavonoids), terpenes, organic acids and hydrogen peroxide. The presence of such substances explains honey’s biological activity and absorbance of ultraviolet radiation. Honey is one of the three most adulterated foods worldwide. High-complexity and sensitivity techniques are used to detect such adulterations, like isotopic analysis and Nuclear Magnetic Resonance. However, a simpler, high-speed, low cost and more accessible analytical method would be more convenient to perform food analyses, particularly for bee products. In this regard, UV-Vis spectroscopy coupled with chemometric analysis has been widely and successfully used to analyze bee products. Therefore, this study aims to validate a methodology based on the use of UV-Vis spectroscopy coupled with chemometric analysis to differentiate the nectar recently collected by bees from partially mature honey and honey. Samples of different geographical and botanical origins were collected from two locations in Argentina; one trial was carried out in Tucumán (in the main lemon flow) and the other in Entre Ríos (eucalyptus flowering). Spectra between 190 nm and 420 nm were recorded and different projection methods were applied, including principal component analysis, FreeViz, and linear discriminant analysis. From the results, it is concluded that UV-Vis spectroscopy coupled with chemometric analysis allows differentiating recently collected nectar from partially mature honey and honey and that phenolic compounds are highly present in mature honey, which could favourably affect its biological activity. 2023 artículo científico 0325-8718 https://www.redalyc.org/articulo.oa?id=86476450004 https://www.redalyc.org/journal/864/86476450004/ https://www.redalyc.org/journal/864/86476450004/html/ https://www.redalyc.org/journal/864/86476450004/86476450004.epub https://www.redalyc.org/journal/864/86476450004/movil https://doi.org/10.58149/wtmq-vz93 en http://www.redalyc.org/revista.oa?id=864 RIA. Revista de Investigaciones Agropecuarias application/pdf Instituto Nacional de Tecnología Agropecuaria RIA. Revista de Investigaciones Agropecuarias (Argentina) Num.3 Vol.49 |
| format | Artículo científico |
| id | redalyc_86476450004 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Instituto Nacional de Tecnología Agropecuaria |
| spellingShingle | UV-Vis spectroscopy and chemometric: a simple method to differentiate honey, partially ripe honey and nectars L. M. Maldonado Agrociencias UV Honey nectar chemometrics Vis spectroscopy UV-Vis spectroscopy and chemometric: a simple method to differentiate honey, partially ripe honey and nectars L. M. Maldonado K. Marcinkevicius A.R. Álvarez V.M. Salomón M. Gaggiotti N. Messina M.A. Palacio S. Medina M.M. Maldonado E. Orellano E. L. Bedascarrasbure Agrociencias UV Honey nectar chemometrics Vis spectroscopy Honey is a supersaturated solution of sugars that also contains small amounts of enzymes, vitamins, phenolic compounds (e.g., flavonoids), terpenes, organic acids and hydrogen peroxide. The presence of such substances explains honey’s biological activity and absorbance of ultraviolet radiation. Honey is one of the three most adulterated foods worldwide. High-complexity and sensitivity techniques are used to detect such adulterations, like isotopic analysis and Nuclear Magnetic Resonance. However, a simpler, high-speed, low cost and more accessible analytical method would be more convenient to perform food analyses, particularly for bee products. In this regard, UV-Vis spectroscopy coupled with chemometric analysis has been widely and successfully used to analyze bee products. Therefore, this study aims to validate a methodology based on the use of UV-Vis spectroscopy coupled with chemometric analysis to differentiate the nectar recently collected by bees from partially mature honey and honey. Samples of different geographical and botanical origins were collected from two locations in Argentina; one trial was carried out in Tucumán (in the main lemon flow) and the other in Entre Ríos (eucalyptus flowering). Spectra between 190 nm and 420 nm were recorded and different projection methods were applied, including principal component analysis, FreeViz, and linear discriminant analysis. From the results, it is concluded that UV-Vis spectroscopy coupled with chemometric analysis allows differentiating recently collected nectar from partially mature honey and honey and that phenolic compounds are highly present in mature honey, which could favourably affect its biological activity. 2023 artículo científico 0325-8718 https://www.redalyc.org/articulo.oa?id=86476450004 https://www.redalyc.org/journal/864/86476450004/ https://www.redalyc.org/journal/864/86476450004/html/ https://www.redalyc.org/journal/864/86476450004/86476450004.epub https://www.redalyc.org/journal/864/86476450004/movil https://doi.org/10.58149/wtmq-vz93 en http://www.redalyc.org/revista.oa?id=864 RIA. Revista de Investigaciones Agropecuarias application/pdf Instituto Nacional de Tecnología Agropecuaria RIA. Revista de Investigaciones Agropecuarias (Argentina) Num.3 Vol.49 |
| title | UV-Vis spectroscopy and chemometric: a simple method to differentiate honey, partially ripe honey and nectars |
| topic | Agrociencias UV Honey nectar chemometrics Vis spectroscopy |
| url | https://www.redalyc.org/articulo.oa?id=86476450004 https://www.redalyc.org/journal/864/86476450004/ https://www.redalyc.org/journal/864/86476450004/html/ https://www.redalyc.org/journal/864/86476450004/86476450004.epub https://www.redalyc.org/journal/864/86476450004/movil https://doi.org/10.58149/wtmq-vz93 |