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| Format: | Artículo científico |
| Language: | en |
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Asociación Española de Toxicología
2014
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| Online Access: | https://www.redalyc.org/articulo.oa?id=91932969015 |
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Table of Contents:
- Cytotoxic and interactive effects of zearalenone, a-zearalenol and b-zearalenol and formation of metabolites in HepG2 cells E Tatay G Meca G Font MJ Ruiz Medicina isobolas zearalenone HepG2 cells metabolites interactive effect Zearalenone (ZEA) is a secondary metabolite of Fusarium fungi. ZEA is a non-steroidal estrogenic mycotoxin which is rapidly absorbed and metabolized to α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in the liver; therefore mixtures of these mycotoxins may be simultaneously present in biological systems and cause human health risk. The objectives of this study were: a) to compare the cytotoxicity of ZEA, α-ZOL and β-ZOL alone or in combination on human hepatoma (HepG2) cells using the MTT assay after 24, 48 and 72h of exposure, and b) to evaluate the interactions of these mycotoxins mixtures in HepG2 cell lines by the isobologram analysis. The IC50, values obtained for individual mycotoxins range from 70.0 to > 100.0 µM, from 20.6 to 26.0 µM and from 38.4 to > 100.0 µM in HepG2 cells for ZEA, α-ZOL and β-ZOL, respectively. Isobologram analysis provides a combination index (CI) value to determine the type of interaction that occurs. The ínterections of ZEA and its metabolites showed slightly synergism (CI from 0.34 ± 0.10 to 0.69 ± 0.22) followed by additive effect (CI from 0.97 ± 0.20 to 2.6 1 ± 2.15) and turned into antagonism (CI from 1.29 ± 0.18 to 7.77 ± 2.27). The concentration of ZEA and its metabolites was determined with liquid chromatography coupled to the rnass spectrometer detector-linear ion trap (LC-MS-LIT). No conversion of ZEA in α-ZOL and β-ZOL was detected. However other degradation products were detected. 2014 artículo científico 0212-7113 https://www.redalyc.org/articulo.oa?id=91932969015 en http://www.redalyc.org/revista.oa?id=919 Revista de Toxicología application/pdf Asociación Española de Toxicología Revista de Toxicología (España) Num.2 Vol.31