Kinetic of the enzymatic degradation of chitosan using bromelain: a viscosimetric study
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Universidad de los Andes
2007
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| _version_ | 1876451448611405824 |
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| author | Cristóbal Lárez Velásquez |
| author_facet | Cristóbal Lárez Velásquez |
| contents | Kinetic of the enzymatic degradation of chitosan using bromelain: a viscosimetric study Cristóbal Lárez Velásquez Dimas Medina Enrique Millán Carlos Torres Química bromelain degradation studies chitosan depolymerization enzymatic depolymerization Enzymatic degradation of chitosan (degree of acetylation (da) and average viscosimetric molecular weight (MV) of0.85 and 310 kDa, respectively) was carried out using commercial bromelain. Decrease of the average viscosimetricpolymerization degree (XV) was studied in a buffer solution of acetic acid (0.3 M)/sodium acetate (0.2 M) at 25 ºC.Under these experimental conditions it is possible to relate the instantaneous intrinsic viscosity [η] with MV throughthe Mark-Houwink-Sakurada equation. Equations of Sano, Solomon-Ciuta, Deb-Chatterjee and Ram-Yaseen, whichallow to compute values of [η] with a single measurement of relative (ηrel) and/or specific viscosity (ηsp), wereemployed to obtain instantaneous values of [η]. Instantaneous values of [η] were also obtained through an iterativeprocess using Huggins equation. Initial velocity of depolymerization (vo) was obtained from different concentrationof chitosan solutions and a fixed bromelain concentration. Michaelis-Menten constant (kM) and maximum velocity(Vm) were obtained using the Lineweaver-Burk representation of the Michaelis-Menten equation for each set of [η]which were calculated employing all procedures mentioned. Best kinetic parameters for chitosan depolymerization(kM=3.92x10-7 mol.dL-1; Vm=9.09x10-8 mol.dL-1.s-1) were obtained when Sano`s equation was employed to calculate[η] values. 2007 artículo científico 1856-5301 https://www.redalyc.org/articulo.oa?id=93320204 en http://www.redalyc.org/revista.oa?id=933 Avances en Química application/pdf Universidad de los Andes Avances en Química (República Bolivariana de Venezuela) Num.2 Vol.2 |
| format | Artículo científico |
| id | redalyc_93320204 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Universidad de los Andes |
| spellingShingle | Kinetic of the enzymatic degradation of chitosan using bromelain: a viscosimetric study Cristóbal Lárez Velásquez Química bromelain degradation studies chitosan depolymerization enzymatic depolymerization Kinetic of the enzymatic degradation of chitosan using bromelain: a viscosimetric study Cristóbal Lárez Velásquez Dimas Medina Enrique Millán Carlos Torres Química bromelain degradation studies chitosan depolymerization enzymatic depolymerization Enzymatic degradation of chitosan (degree of acetylation (da) and average viscosimetric molecular weight (MV) of0.85 and 310 kDa, respectively) was carried out using commercial bromelain. Decrease of the average viscosimetricpolymerization degree (XV) was studied in a buffer solution of acetic acid (0.3 M)/sodium acetate (0.2 M) at 25 ºC.Under these experimental conditions it is possible to relate the instantaneous intrinsic viscosity [η] with MV throughthe Mark-Houwink-Sakurada equation. Equations of Sano, Solomon-Ciuta, Deb-Chatterjee and Ram-Yaseen, whichallow to compute values of [η] with a single measurement of relative (ηrel) and/or specific viscosity (ηsp), wereemployed to obtain instantaneous values of [η]. Instantaneous values of [η] were also obtained through an iterativeprocess using Huggins equation. Initial velocity of depolymerization (vo) was obtained from different concentrationof chitosan solutions and a fixed bromelain concentration. Michaelis-Menten constant (kM) and maximum velocity(Vm) were obtained using the Lineweaver-Burk representation of the Michaelis-Menten equation for each set of [η]which were calculated employing all procedures mentioned. Best kinetic parameters for chitosan depolymerization(kM=3.92x10-7 mol.dL-1; Vm=9.09x10-8 mol.dL-1.s-1) were obtained when Sano`s equation was employed to calculate[η] values. 2007 artículo científico 1856-5301 https://www.redalyc.org/articulo.oa?id=93320204 en http://www.redalyc.org/revista.oa?id=933 Avances en Química application/pdf Universidad de los Andes Avances en Química (República Bolivariana de Venezuela) Num.2 Vol.2 |
| title | Kinetic of the enzymatic degradation of chitosan using bromelain: a viscosimetric study |
| topic | Química bromelain degradation studies chitosan depolymerization enzymatic depolymerization |
| url | https://www.redalyc.org/articulo.oa?id=93320204 |