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1. Verfasser:	Enrique J. Millán Barrios
Format:	Artículo científico
Sprache:	es
Veröffentlicht:	Universidad de los Andes 2015
Schlagworte:	Química nanoscopio Fluorescencia supermicroscopio
Online-Zugang:	https://www.redalyc.org/articulo.oa?id=93341009003
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La nanoscopía, el reto alcanzado de apreciar las células vivas en su interior Enrique J. Millán Barrios Química nanoscopio Fluorescencia supermicroscopio Se presenta una breve descripción del proceso y actores involucrados en el de desarrollo del supermicroscopio de fluorescencia o nanoscopía. Se intenta mostrar, de manera resumida, la contribución de los investigadores involucrados en el desarrollo de esta técnica, lo cual los llevó a ser reconocidos con el premio Nobel de Química. La participación individual de cada investigador aportó lo conocimientos, procesos y resultados experimentales necesarios para lograr observar células de manera individual, al aprovechar la fluorescencia que presentan ciertas moléculas a nivel celular, llegando a obtener una alta resolución a nivel nanométrico. El impacto y nuevos desarrollos, especialmente en el campo de la biología celular y genética, que esta técnica ofrece están por verse en el corto tiempo. 2015 artículo científico 1856-5301 https://www.redalyc.org/articulo.oa?id=93341009003 es http://www.redalyc.org/revista.oa?id=933 Avances en Química application/pdf Universidad de los Andes Avances en Química (República Bolivariana de Venezuela) Num.1 Vol.10

if (filemtime($filename) + $maxlifetime < time()) { unlink($filename); $count++; } } return $count; } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ protected function saveSession($sessId, $data): bool { $sessFile = $this->path . '/sess_' . $sessId; if ($handle = fopen($sessFile, 'w')) { $return = false; // Lock the file for exclusive access to avoid issues with multiple // processes writing session simultaneously: if (flock($handle, LOCK_EX)) { $return = fwrite($handle, $data); // Make sure that there's no trailing data by truncating the file to // the correct length: ftruncate($handle, strlen($data)); fflush($handle); flock($handle, LOCK_UN); } fclose($handle); if ($return !== false) { return true; } } // If we got this far, something went wrong with the file output... // It is tempting to throw an exception here, but this code is called // outside of the context of exception handling, so all we can do is // echo a message.

// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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