Efecto del factor de crecimiento epidermal (EGF) durante la maduración de oocitos sobre la producción in vitro de embriones bovinos
Fuente:
Redalyc
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Universidad del Zulia
2004
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1876455791510159360 |
|---|---|
| author | Eleazar Soto Belloso |
| author_facet | Eleazar Soto Belloso |
| contents | Efecto del factor de crecimiento epidermal (EGF) durante la maduración de oocitos sobre la producción in vitro de embriones bovinos Eleazar Soto Belloso Roberto Palomares Naveda Aitor De Ondíz Sánchez Hugo Hernández Fonseca Rumualdo González Fernández Fernando Perea Ganchou Saksiri Sirisathian Benjamín Brackett Veterinaria bovine maturation of oocytes in vitro fertilization Epidermal growth factor The aim of this experiment was to research the effect of Epidermal Growth Factor (EGF)during in vitro maturation (IVM) of bovine oocytes on the development of bovine embryosin vitro. The oocytes were recovered from ovaries of slaughtered cows. An IVM mediacontaining serum was compared with chemically defined medias. As a result, 163 Cumulusoocyte complexed (COC´s)were selected , with three or more layers of granulosa cells,and were incubated for 24 hours in one of the three maturation medias: 1) Modified OMM(T1); 2) Modified OMM supplemented with 10 ng/ml of EGF (T2); 3) Modified OMMsupplemented with 10% of fetal bovine serum (T3). Following IVM, COC ´s wereinseminated with 2x106 spermatozoa / ml. After 18 hours of gamete coincubation,presumptive zygotes were denuded and placed in culture until the blastocyst stage.Cleavage rates were similar in T2 and T3 , but significantly greater than in T1 (P<0.05).Addition of EGF during in vitro maturation (T2) did not yield more blastocyst compared tothe other treatments. None the less, T3 (with serum) had a greater yield of blastocystscompared with T1 (P<0.01). Results in this study show that the addition of EGF tochemically defined media produces similar cleavage rates and blastocyst yields to thoseobtained when using serum during IVM, representing an alternative for in vitro productionof bovine embryos 2004 artículo científico 0798-2259 https://www.redalyc.org/articulo.oa?id=95914210 en http://www.redalyc.org/revista.oa?id=959 Revista Científica application/pdf Universidad del Zulia Revista Científica (República Bolivariana de Venezuela) Num.2 Vol.XIV |
| format | Artículo científico |
| id | redalyc_95914210 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Universidad del Zulia |
| spellingShingle | Efecto del factor de crecimiento epidermal (EGF) durante la maduración de oocitos sobre la producción in vitro de embriones bovinos Eleazar Soto Belloso Veterinaria bovine maturation of oocytes in vitro fertilization Epidermal growth factor Efecto del factor de crecimiento epidermal (EGF) durante la maduración de oocitos sobre la producción in vitro de embriones bovinos Eleazar Soto Belloso Roberto Palomares Naveda Aitor De Ondíz Sánchez Hugo Hernández Fonseca Rumualdo González Fernández Fernando Perea Ganchou Saksiri Sirisathian Benjamín Brackett Veterinaria bovine maturation of oocytes in vitro fertilization Epidermal growth factor The aim of this experiment was to research the effect of Epidermal Growth Factor (EGF)during in vitro maturation (IVM) of bovine oocytes on the development of bovine embryosin vitro. The oocytes were recovered from ovaries of slaughtered cows. An IVM mediacontaining serum was compared with chemically defined medias. As a result, 163 Cumulusoocyte complexed (COC´s)were selected , with three or more layers of granulosa cells,and were incubated for 24 hours in one of the three maturation medias: 1) Modified OMM(T1); 2) Modified OMM supplemented with 10 ng/ml of EGF (T2); 3) Modified OMMsupplemented with 10% of fetal bovine serum (T3). Following IVM, COC ´s wereinseminated with 2x106 spermatozoa / ml. After 18 hours of gamete coincubation,presumptive zygotes were denuded and placed in culture until the blastocyst stage.Cleavage rates were similar in T2 and T3 , but significantly greater than in T1 (P<0.05).Addition of EGF during in vitro maturation (T2) did not yield more blastocyst compared tothe other treatments. None the less, T3 (with serum) had a greater yield of blastocystscompared with T1 (P<0.01). Results in this study show that the addition of EGF tochemically defined media produces similar cleavage rates and blastocyst yields to thoseobtained when using serum during IVM, representing an alternative for in vitro productionof bovine embryos 2004 artículo científico 0798-2259 https://www.redalyc.org/articulo.oa?id=95914210 en http://www.redalyc.org/revista.oa?id=959 Revista Científica application/pdf Universidad del Zulia Revista Científica (República Bolivariana de Venezuela) Num.2 Vol.XIV |
| title | Efecto del factor de crecimiento epidermal (EGF) durante la maduración de oocitos sobre la producción in vitro de embriones bovinos |
| topic | Veterinaria bovine maturation of oocytes in vitro fertilization Epidermal growth factor |
| url | https://www.redalyc.org/articulo.oa?id=95914210 |