Localizable Fluorescent Metal Ion Indicators With Tunable Colors
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Wiley Open Access
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| Format: | Artículo Open Access |
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Wiley
2026
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| author | Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson |
| author_facet | Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson |
| collection | Wiley Open Access |
| contents | Localizable Fluorescent Metal Ion Indicators With Tunable Colors Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson Advanced Science ABSTRACT Elucidating the role of metal ion homeostasis in physiological and pathological processes requires detection tools with high sensitivity and selectivity, spectral versatility, and precise subcellular localization. Here, we introduce a modular strategy for generating fluorescent metal ion indicators that are comprised of a sulfonamide‐functionalized chelator, a rhodamine derivative, and a ligand for bioconjugation to self‐labeling proteins. With a concise three‐step synthesis, the design allows tuning of both the emission color and ligand specificity of the resulting indicators. Specifically, we developed a family of bright, color‐tunable potassium indicators that can be selectively coupled to intra‐ or extracellular HaloTag and SNAP‐tag fusion proteins. The increase in fluorescence upon binding to HaloTag or SNAP‐tag enabled wash‐free live‐cell imaging. The localized potassium indicators enabled the detection of dynamic potassium efflux in rat hippocampal neurons upon glutamate stimulation. Our work thus establishes a versatile platform for the generation of localizable fluorescent metal ion indicators and opens up new avenues for live‐cell potassium sensing. 10.1002/advs.75402 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1002/advs.75402 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_advs_75402 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2026 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Localizable Fluorescent Metal Ion Indicators With Tunable Colors Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson Advanced Science Localizable Fluorescent Metal Ion Indicators With Tunable Colors Ming‐Ming Wang De‐en Sun Klara Gries Kai Johnsson Advanced Science ABSTRACT Elucidating the role of metal ion homeostasis in physiological and pathological processes requires detection tools with high sensitivity and selectivity, spectral versatility, and precise subcellular localization. Here, we introduce a modular strategy for generating fluorescent metal ion indicators that are comprised of a sulfonamide‐functionalized chelator, a rhodamine derivative, and a ligand for bioconjugation to self‐labeling proteins. With a concise three‐step synthesis, the design allows tuning of both the emission color and ligand specificity of the resulting indicators. Specifically, we developed a family of bright, color‐tunable potassium indicators that can be selectively coupled to intra‐ or extracellular HaloTag and SNAP‐tag fusion proteins. The increase in fluorescence upon binding to HaloTag or SNAP‐tag enabled wash‐free live‐cell imaging. The localized potassium indicators enabled the detection of dynamic potassium efflux in rat hippocampal neurons upon glutamate stimulation. Our work thus establishes a versatile platform for the generation of localizable fluorescent metal ion indicators and opens up new avenues for live‐cell potassium sensing. 10.1002/advs.75402 http://creativecommons.org/licenses/by/4.0/ |
| title | Localizable Fluorescent Metal Ion Indicators With Tunable Colors |
| topic | Advanced Science |
| url | https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.75402 |