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| Main Authors: | , , , , , , |
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| Format: | Artículo Open Access |
| Published: |
Wiley
2025
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| Online Access: | https://onlinelibrary.wiley.com/doi/10.1002/app.57430 |
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Table of Contents:
- Diabetic Wound Healing Using Momordica charantia Extract‐Mediated Silver Nanoparticles Loaded on Electrospun Nanofibrous Wound Dressing Guihua Liang Gaurav Sanghvi R. Roopashree Aditya Kashyap Shaker Al‐Hasnaawei D. Alex Anand Rajashree Panigrahi Journal of Applied Polymer Science ABSTRACTThe main aim of the current assay was to fabricate bioactive/interactive nanofibrous wound dressings with cytocompatibility, antibacterial, and antioxidant properties. Phytosynthesized silver nanoparticles (AgNPs) mediated via Momordica charantia extract (as the reducing and capping agent) were applied into polycaprolactone (PCL) electrospun nanofibers. Using scanning electron microscopy (SEM), transmission electron microscope (TEM), DLS, Zeta potential, energy‐dispersive X‐ray spectrometer (EDX), and X‐ray diffraction (XRD), we confirmed the phytosynthesis of AgNPs. Using SEM, we observed the morphology of PCL and PCL/AgNPs nanofibers, and the presence of AgNPs was confirmed. Characterization techniques showed that the fabricated nanofibrous wound dressings have a porous microstructure (with porosity in the range of 50%–60%), and PCL/AgNPs nanofibers exhibited acceptable hydrophilicity (with water contact angel degree of 53.0° ± 2.5°). The mean diameter of the unaltered polyurethane fibers was found to be 252 ± 55 nm, while the PCL nanofibers including AgNPs had an average diameter of 305 ± 29 nm. The biological evaluations showed that the fabricated nanofibrous wound dressings were cytocompatible (with toxicity of less than 10%) and exhibited antioxidant (dose dependent) and antibacterial activities. The WVTR for the control group (open container) was 46.0 ± 5.6 mg/cm2 h, while the WVTR values for PCL nanofibers and PCL/AgNPs nanofibers were 8.3 ± 2.5 mg/cm2 h and 8.6 ± 3.5 mg/cm2 h, respectively. The hemolysis assay showed that the PCL nanofibers exhibited a hemolysis value of 14.3% ± 2.5%, and PCL/AgNPs exhibited a hemolysis value of 8.0% ± 3.1%. In vivo wound healing in streptozotocin (STZ)‐induced diabetes in the rat showed that applying the fabricated PCL/AgNPs nanofibers accelerated wound healing and induced a fully repaired wound. In contrast, the wounds of diabetic rats that received treatment with pure mats did not exhibit complete healing. Moreover, the PCL/AgNPs nanofibers increased hexosamine, collagen, and hydroxyproline contents and subsequently elevated HIF‐1α, TGF‐β1, and VEGF (angiogenesis factors contents). Thus, it can be inferred that utilizing the nanofibrous mat incorporating AgNPs may be deemed an appropriate intervention for wound dressing. This intervention can potentially expedite the healing duration and mitigate the risk of infection along the wound healing trajectory. 10.1002/app.57430 http://onlinelibrary.wiley.com/termsAndConditions#vor