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| Main Authors: | , , , |
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| Format: | Artículo Open Access |
| Published: |
Wiley
2025
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| Subjects: | |
| Online Access: | https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/aps3.70004 |
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Table of Contents:
- A low‐cost protocol for the optical method of vulnerability curves to calculate P50 Georgina González‐Rebeles Miguel Ángel Alonso‐Arevalo Eulogio López Rodrigo Méndez‐Alonzo Applications in Plant Sciences AbstractPremiseThe quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost‐effective protocol to measure the water potential at which 50% of hydraulic conductivity (P50) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.Methods and ResultsOur protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify P50 in an exotic species (Nicotiana glauca) and native species (Rhus integrifolia) of the Mediterranean vegetation in Baja California, Mexico.ConclusionsThe intra‐ and interspecific patterns of variation in stem P50 of N. glauca and R. integrifolia were obtained using the low‐cost optical method with widely available and affordable materials that can be easily replicated for other species. 10.1002/aps3.70004 http://creativecommons.org/licenses/by/4.0/