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| Main Authors: | , , , , , , , |
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| Format: | Artículo Open Access |
| Published: |
Wiley
2024
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| Subjects: | |
| Online Access: | https://onlinelibrary.wiley.com/doi/10.1002/arch.22113 |
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Table of Contents:
- RNAi efficiency is enhanced through knockdown of double‐stranded RNA‐degrading enzymes in butterfly Papilio xuthus Qian Shu Gui‐chun Liu Jin‐wu He Ping Hu Zhi‐wei Dong Ruo‐ping Zhao Hong‐rui Zhang Xue‐yan Li Archives of Insect Biochemistry and Physiology AbstractThe efficiency of RNA interference (RNAi) has always limited the research on the phenotype innovation of Lepidoptera insects. Previous studies have found that double‐stranded RNA‐degrading enzyme (dsRNase) is an important factor in RNAi efficiency, but there have been no relevant reports in butterflies (Papilionoidea). Papilio xuthus is one of the important models in butterflies with an extensive experimental application value. To explore the effect of dsRNase in the RNAi efficiency on butterflies, six dsRNase genes (PxdsRNase 1–6) were identified in P. xuthus genome, and their dsRNA‐degrading activities were subsequently detected by ex vivo assays. The result shows that the dsRNA‐degrading ability of gut content (<1 h) was higher than hemolymph content (>12 h). We then investigated the expression patterns of these PxdsRNase genes during different tissues and developmental stages, and related RNAi experiments were carried out. Our results show that different PxdsRNase genes had different expression levels at different developmental stages and tissues. The expression of PxdsRNase2, PxdsRNase3, and PxdsRNase6 were upregulated significantly through dsGFP injection, and PxdsRNase genes can be silenced effectively by injecting their corresponding dsRNA. RNAi‐of‐RNAi studies with PxEbony, which acts as a reporter gene, observed that silencing PxdsRNase genes can increase RNAi efficiency significantly. These results confirm that silencing dsRNase genes can improve RNAi efficiency in P. xuthus significantly, providing a reference for the functional study of insects such as butterflies with low RNAi efficiency. 10.1002/arch.22113 http://onlinelibrary.wiley.com/termsAndConditions#vor