Pelargonidin inhibits cell growth and promotes oxidative stress‐mediated apoptosis in lung cancer A549 cells
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| Format: | Artículo Open Access |
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2024
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| author | Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi |
| author_facet | Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi |
| collection | Wiley Open Access |
| contents | Pelargonidin inhibits cell growth and promotes oxidative stress‐mediated apoptosis in lung cancer A549 cells Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi Biotechnology and Applied Biochemistry Abstract Lung cancer has the worst prognosis with an average 5‐year survival rate of only 10%–20%. Lung cancer has the highest prevalence rate and a second most common cause of cancer‐associated mortalities worldwide. The present study was planned to explore the anticancer effects of pelargonidin against the lung cancer A549 cells via analyzing oxidative stress‐mediated apoptosis. The viability of both control and pelargonidin‐treated A549 cells was analyzed using the MTT cytotoxicity assay at different time periods. The levels of endogenous ROS generation, mitochondrial membrane potential (Δ ψ m ), and apoptosis were assessed using corresponding fluorescent staining assays. The levels of oxidative stress biomarkers, including TBARS, SOD, CAT, and GSH, in the cell lysates of control and pelargonidin‐treated A549 cells were examined using the assay kits. The pelargonidin treatment substantially suppressed the A549 cell growth. Further, pelargonidin promoted the ROS production and depleted the Δ ψ m levels in the A549 cells. The fluorescent staining assays witnessed the occurrence of increased apoptosis in the pelargonidin‐treated A549 cells. The pelargonidin also boosted the TBARS and reduced the antioxidant levels thereby promoted the oxidative stress‐regulated apoptosis in the A549 cells. In summary, the findings’ results of the current study demonstrated an anticancer activity of pelargonidin on A549 cells. The pelargonidin treatment substantially decreased the growth and encouraged the oxidative stress‐regulated apoptosis in A549 cells. Therefore, it was evident that the pelargonidin could be employed as an effective anticancer candidate to treat the lung cancer. 10.1002/bab.2621 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/bab.2621 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_bab_2621 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Pelargonidin inhibits cell growth and promotes oxidative stress‐mediated apoptosis in lung cancer A549 cells Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi Biotechnology and Applied Biochemistry Pelargonidin inhibits cell growth and promotes oxidative stress‐mediated apoptosis in lung cancer A549 cells Liwei Yue Ying Li Yuting Luo Abdullah A. Alarfaj Yubo Shi Biotechnology and Applied Biochemistry Abstract Lung cancer has the worst prognosis with an average 5‐year survival rate of only 10%–20%. Lung cancer has the highest prevalence rate and a second most common cause of cancer‐associated mortalities worldwide. The present study was planned to explore the anticancer effects of pelargonidin against the lung cancer A549 cells via analyzing oxidative stress‐mediated apoptosis. The viability of both control and pelargonidin‐treated A549 cells was analyzed using the MTT cytotoxicity assay at different time periods. The levels of endogenous ROS generation, mitochondrial membrane potential (Δ ψ m ), and apoptosis were assessed using corresponding fluorescent staining assays. The levels of oxidative stress biomarkers, including TBARS, SOD, CAT, and GSH, in the cell lysates of control and pelargonidin‐treated A549 cells were examined using the assay kits. The pelargonidin treatment substantially suppressed the A549 cell growth. Further, pelargonidin promoted the ROS production and depleted the Δ ψ m levels in the A549 cells. The fluorescent staining assays witnessed the occurrence of increased apoptosis in the pelargonidin‐treated A549 cells. The pelargonidin also boosted the TBARS and reduced the antioxidant levels thereby promoted the oxidative stress‐regulated apoptosis in the A549 cells. In summary, the findings’ results of the current study demonstrated an anticancer activity of pelargonidin on A549 cells. The pelargonidin treatment substantially decreased the growth and encouraged the oxidative stress‐regulated apoptosis in A549 cells. Therefore, it was evident that the pelargonidin could be employed as an effective anticancer candidate to treat the lung cancer. 10.1002/bab.2621 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Pelargonidin inhibits cell growth and promotes oxidative stress‐mediated apoptosis in lung cancer A549 cells |
| topic | Biotechnology and Applied Biochemistry |
| url | https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2621 |