Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus
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| Format: | Artículo Open Access |
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Wiley
2025
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| _version_ | 1867010810991083520 |
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| author | Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita |
| author_facet | Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita |
| collection | Wiley Open Access |
| contents | Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita Biotechnology and Bioengineering ABSTRACT Freestanding cell‐sheets are valuable bio‐materials for use in regenerative medicine and tissue engineering. Because cell‐sheets experience various mechanical stimulations during handling, it is important to understand the responses of cells to these stimulations. Here, we demonstrate changes in the localization of various proteins during the stretching of fibroblast cell‐sheets. These proteins are known to be involved in mechano‐sensing. Upon stretching, actin filaments appear parallel to the stretching direction. At cell‐cell junctions, β‐catenin forms clusters that co‐localize with accumulated vinculin and zyxin as well as the actin filaments. The p130 Crk‐associated substrate, known to be present in focal adhesions, is also recruited to these clusters and phosphorylated. Our results suggest that mechano‐sensing machinery is formed at cell‐cell junctions when the cell‐sheets are stretched. 10.1002/bit.28996 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1002/bit.28996 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_bit_28996 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita Biotechnology and Bioengineering Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus Madoka Suzuki Keiko Kawauchi Hiroaki Machiyama Hiroaki Hirata Shin'ichi Ishiwata Hideaki Fujita Biotechnology and Bioengineering ABSTRACT Freestanding cell‐sheets are valuable bio‐materials for use in regenerative medicine and tissue engineering. Because cell‐sheets experience various mechanical stimulations during handling, it is important to understand the responses of cells to these stimulations. Here, we demonstrate changes in the localization of various proteins during the stretching of fibroblast cell‐sheets. These proteins are known to be involved in mechano‐sensing. Upon stretching, actin filaments appear parallel to the stretching direction. At cell‐cell junctions, β‐catenin forms clusters that co‐localize with accumulated vinculin and zyxin as well as the actin filaments. The p130 Crk‐associated substrate, known to be present in focal adhesions, is also recruited to these clusters and phosphorylated. Our results suggest that mechano‐sensing machinery is formed at cell‐cell junctions when the cell‐sheets are stretched. 10.1002/bit.28996 http://creativecommons.org/licenses/by/4.0/ |
| title | Dynamic Remodeling of Mechano‐Sensing Complexes in Suspended Fibroblast Cell‐Sheets Under External Mechanical Stimulus |
| topic | Biotechnology and Bioengineering |
| url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.28996 |