Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi
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| Format: | Artículo Open Access |
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Wiley
2025
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| author | Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng |
| author_facet | Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng |
| collection | Wiley Open Access |
| contents | Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng Biotechnology and Bioengineering ABSTRACTGibberellic acid (GA3), a quintessential diterpenoid phytohormone, is indispensable in agronomic practices, horticulture, and the wine industry. This study implemented a combinatorial metabolic engineering strategy within Fusarium fujikuroi (F. fujikuroi) by integrating the potentiation of global regulatory factors (GRFs), and amplification of biosynthetic precursors, alongside dynamic modulation of cofactors with dissolved oxygen supply, to precisely enhance GA3 biosynthesis. Transcriptomic analyses revealed that positive GRFs (AreB, Hat1, and Ada3) enhanced carbon and nitrogen metabolism, increased biomass accumulation, and upregulated transcription levels of the GA3 biosynthetic gene cluster. The use of endogenous nitrogen‐responsive promoters ensured a balanced supply of cofactors and oxygen, thereby preventing the accumulation of terpenoid by‐products. These combinatorial metabolic engineering strategies presented in this study make a significant step toward the enhancement of GA3 yield (3.22 g/L) via submerged fermentation of F. fujikuroi, offering novel insights to enable high‐level biosynthesis of secondary metabolites in fungal chassis. 10.1002/bit.29005 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/bit.29005 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_bit_29005 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng Biotechnology and Bioengineering Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi Xia Ke Yao Chen Rui Jia Hao‐Nan Wang Zhi‐Qiang Liu Yu‐Guo Zheng Biotechnology and Bioengineering ABSTRACTGibberellic acid (GA3), a quintessential diterpenoid phytohormone, is indispensable in agronomic practices, horticulture, and the wine industry. This study implemented a combinatorial metabolic engineering strategy within Fusarium fujikuroi (F. fujikuroi) by integrating the potentiation of global regulatory factors (GRFs), and amplification of biosynthetic precursors, alongside dynamic modulation of cofactors with dissolved oxygen supply, to precisely enhance GA3 biosynthesis. Transcriptomic analyses revealed that positive GRFs (AreB, Hat1, and Ada3) enhanced carbon and nitrogen metabolism, increased biomass accumulation, and upregulated transcription levels of the GA3 biosynthetic gene cluster. The use of endogenous nitrogen‐responsive promoters ensured a balanced supply of cofactors and oxygen, thereby preventing the accumulation of terpenoid by‐products. These combinatorial metabolic engineering strategies presented in this study make a significant step toward the enhancement of GA3 yield (3.22 g/L) via submerged fermentation of F. fujikuroi, offering novel insights to enable high‐level biosynthesis of secondary metabolites in fungal chassis. 10.1002/bit.29005 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi |
| topic | Biotechnology and Bioengineering |
| url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.29005 |