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| Main Authors: | , , , , |
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| Format: | Artículo Open Access |
| Published: |
Wiley
2026
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| Subjects: | |
| Online Access: | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.70246 |
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Table of Contents:
- Resilient High‐Rate Sulfidogenesis in a Hydrogen‐Based Membrane Biofilm Reactor: Mechanistic Analysis of Kinetic‐Limited Performance Under Variable Loading José Suárez Sebastián Roa Vicente Valdés Iván Nancucheo Alex Schwarz Biotechnology and Bioengineering ABSTRACT Copper mining industry generates water with high sulfate (SO 4 2− ) concentrations. The hydrogen‐based membrane biofilm reactor (H 2 ‐MBfR) is a promising sulfidogenesis solution, using H 2 as a clean electron donor. An H 2 ‐MBfR was operated for 209 days treating synthetic mining‐influenced water under varying conditions (HRT, H 2 pressure, SO 4 2− loadings). The system proved highly resilient, achieving stable SO 4 2− removal (> 90%) and fully recovering from a severe shock load (4500 mg S L −1 ). To identify the governing mechanisms, a transient pseudoanalytical biofilm model was developed. A Global Sensitivity Analysis (Sobol) showed performance is dominated by the maximum specific growth rate (), detachment coefficient (), and H 2 S inhibition (). The model was calibrated on Stages 1–3 (Days 0–169, n = 44), achieving R² = 0.962 (calibration) and R² = 0.572 (blind validation, Stage 4). Crucial findings from the model calibration ( = 0.344 d −1 ) and a diagnostic analysis using the Thiele Modulus (mean = 0.224) strongly indicate that the system operated in a kinetically‐limited regime. Performance was governed by the delicate balance of slow growth and high detachment, not by mass transfer (H 2 supply or SO 4 2− diffusion). This research validates the H 2 ‐MBfR for high‐rate applications and correctly shifts the bottleneck for future optimization from mass transfer to biofilm retention kinetics. 10.1002/bit.70246 http://onlinelibrary.wiley.com/termsAndConditions#vor