Cover Image

Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting

Fuente: Wiley Open Access

Saved in:

Bibliographic Details
Main Authors:	Lina Chakrabarti, James Savery, John Patrick Mpindi, Judith Klover, Lina Li, Jie Zhu
Format:	Artículo Open Access
Published:	Wiley 2024
Subjects:	Biotechnology Progress
Online Access:	Acceder al recurso
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
by: Peter Amaya, et al.
Published: (2025)

NS0 and CHO cell lines generate distinct quality profiles for non‐originator NISTmAbs
by: Xin Bush, et al.
Published: (2026)

Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived CHO cell line
by: Theodore W. Peters, et al.
Published: (2026)

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO platforms for cell line development
by: Cintia Gomez Limia, et al.
Published: (2026)

Host cell proteins in monoclonal antibody processing: Control, detection, and removal
by: Takao Ito, et al.
Published: (2024)

N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors
by: Lucas Lemire, et al.
Published: (2025)

Gene copy number, gene configuration and LC / HC mRNA ratio impact on antibody productivity and product quality in targeted integration CHO cell lines
by: Zion Lee, et al.
Published: (2024)

Scale‐up of a monoclonal antibody CHO fed‐batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen
by: Lucas Lemire, et al.
Published: (2025)

Rapid development of a transferable R aman model using high‐throughput cell culture for monitoring monoclonal antibody titer
by: Alexandra Umprecht, et al.
Published: (2026)

Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange
by: Rigumula Wu, et al.
Published: (2024)

Augmenting therapeutic protein production in CHO cells: A proline‐based selection strategy for enhanced productivity and product quality
by: Bin Zhao, et al.
Published: (2025)

Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation
by: Cynthia Lam, et al.
Published: (2024)

Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and 1 H NMR ‐based consumption profiling
by: Shanshan Xu, et al.
Published: (2025)

A deep‐well plate enabled automated high‐throughput cell line development platform
by: Xiaoyan Tang, et al.
Published: (2024)

A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process
by: Shreya Shah, et al.
Published: (2026)

5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5
by: Wenjin Xi, et al.
Published: (2025)

Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing
by: Tiffany Hsu, et al.
Published: (2024)

Correction to “Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in CHO cells via downregulation of PDGFRa and IRE1a signaling”
Published: (2025)

An efficient and robust strategy for developing cell lines through targeted integration
by: Duncan McVey, et al.
Published: (2026)

Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles
by: Jashwant Kumar, et al.
Published: (2025)

Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based gene therapy
by: Yixuan Ming, et al.
Published: (2025)

Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines
by: Niklas Krämer, et al.
Published: (2025)

Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures
by: Sophie Lipshutz, et al.
Published: (2025)

A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines
by: Yu‐Chieh Lin, et al.
Published: (2025)

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth
by: Han‐Jung Kuo, et al.
Published: (2025)

Data‐driven design of deterministic lateral displacement microfluidic devices for CTC separation
by: Tanbir Sarowar, et al.
Published: (2026)

Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on cell health
by: Nithya Krishnan, et al.
Published: (2025)

Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells
by: Yiwen Jiang, et al.
Published: (2025)

Evaluation of two transposases for improving expression of recombinant proteins in Chinese hamster ovary cell stable pools by co‐transfection and supertransfection approaches
by: Melina Lenser, et al.
Published: (2024)

Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture
by: Célia Sanchez, et al.
Published: (2025)

Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography
by: Lalita Kanwar Shekhawat, et al.
Published: (2025)

Biotechnological potential of yeast cell wall: An overview
by: Fanny Machado Jofre, et al.
Published: (2024)

A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy
by: Joseph R. Egan, et al.
Published: (2025)

Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations
by: Jort J. Altenburg, et al.
Published: (2025)

Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic
by: Paras Sharma, et al.
Published: (2025)

Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification
by: Victor Pasquier, et al.
Published: (2024)

Just passing through: Deploying aquaporins in microbial cell factories
by: Liam Richard Jenkins Sánchez, et al.
Published: (2024)

Machine learning‐assisted clone selection for intensified cell culture processes
by: Nicolas Wolnick, et al.
Published: (2026)

A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N , a new regenerated cellulose virus removal filter
by: Asami Inoue, et al.
Published: (2025)

Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes
by: Marina Y. Linova, et al.
Published: (2025)