Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations
Fuente:
Wiley Open Access
Saved in:
| Main Authors: | Brian Ladd, Torbjörn Gräslund, Véronique Chotteau |
|---|---|
| Format: | Artículo Open Access |
| Published: |
Wiley
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based gene therapy
by: Yixuan Ming, et al.
Published: (2025)
by: Yixuan Ming, et al.
Published: (2025)
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines
by: Yu‐Chieh Lin, et al.
Published: (2025)
by: Yu‐Chieh Lin, et al.
Published: (2025)
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth
by: Han‐Jung Kuo, et al.
Published: (2025)
by: Han‐Jung Kuo, et al.
Published: (2025)
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells
by: Min Lu, et al.
Published: (2024)
by: Min Lu, et al.
Published: (2024)
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines
by: Niklas Krämer, et al.
Published: (2025)
by: Niklas Krämer, et al.
Published: (2025)
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions
by: Louis Coplan, et al.
Published: (2024)
by: Louis Coplan, et al.
Published: (2024)
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing
by: Leon P. Pybus, et al.
Published: (2024)
by: Leon P. Pybus, et al.
Published: (2024)
Improved methodology for direct monitoring of large‐scale manufacturing cell culture using Raman spectroscopy data from process development
by: Hadi El Radi, et al.
Published: (2026)
by: Hadi El Radi, et al.
Published: (2026)
Augmenting therapeutic protein production in CHO cells: A proline‐based selection strategy for enhanced productivity and product quality
by: Bin Zhao, et al.
Published: (2025)
by: Bin Zhao, et al.
Published: (2025)
An efficient and robust strategy for developing cell lines through targeted integration
by: Duncan McVey, et al.
Published: (2026)
by: Duncan McVey, et al.
Published: (2026)
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes
by: Marina Y. Linova, et al.
Published: (2025)
by: Marina Y. Linova, et al.
Published: (2025)
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry
by: Fernando Teodoro, et al.
Published: (2025)
by: Fernando Teodoro, et al.
Published: (2025)
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation
by: Fatma Zehra Erkoc‐Biradli, et al.
Published: (2024)
by: Fatma Zehra Erkoc‐Biradli, et al.
Published: (2024)
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing
by: Shyam Panjwani, et al.
Published: (2025)
by: Shyam Panjwani, et al.
Published: (2025)
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing
by: Darrick Shen, et al.
Published: (2024)
by: Darrick Shen, et al.
Published: (2024)
Optimizing cryopreservation strategies for scalable cell therapies: A comprehensive review with insights from iPSC ‐derived therapies
by: Michael Dobruskin, et al.
Published: (2024)
by: Michael Dobruskin, et al.
Published: (2024)
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic
by: Hong Li, et al.
Published: (2024)
by: Hong Li, et al.
Published: (2024)
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO platforms for cell line development
by: Cintia Gomez Limia, et al.
Published: (2026)
by: Cintia Gomez Limia, et al.
Published: (2026)
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories
by: Marcus Wäneskog, et al.
Published: (2024)
by: Marcus Wäneskog, et al.
Published: (2024)
Evaluating the impact of ionic strength on retention of minute virus of mice during small virus‐retentive filtration
by: Cody Murphy, et al.
Published: (2026)
by: Cody Murphy, et al.
Published: (2026)
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing
by: Tiffany Hsu, et al.
Published: (2024)
by: Tiffany Hsu, et al.
Published: (2024)
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150
by: Christopher B. Glascock, et al.
Published: (2024)
by: Christopher B. Glascock, et al.
Published: (2024)
Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange
by: Rigumula Wu, et al.
Published: (2024)
by: Rigumula Wu, et al.
Published: (2024)
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography
by: Lalita Kanwar Shekhawat, et al.
Published: (2025)
by: Lalita Kanwar Shekhawat, et al.
Published: (2025)
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives
by: Catarina P. G. Neves, et al.
Published: (2024)
by: Catarina P. G. Neves, et al.
Published: (2024)
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process
by: Mario Grünberg, et al.
Published: (2026)
by: Mario Grünberg, et al.
Published: (2026)
Use of live digital twin (shadow) soft sensor to monitor membrane degradation in continuous manufacturing single pass tangential flow filtration
by: Robert Taylor, et al.
Published: (2025)
by: Robert Taylor, et al.
Published: (2025)
Control strategy for biopharmaceutical production by model predictive control
by: Touraj Eslami, et al.
Published: (2024)
by: Touraj Eslami, et al.
Published: (2024)
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes
by: Daphne Keulen, et al.
Published: (2024)
by: Daphne Keulen, et al.
Published: (2024)
General strategies for IgG‐like bispecific antibody purification
by: Yifeng Li
Published: (2024)
by: Yifeng Li
Published: (2024)
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies
by: Katherine Forrester, et al.
Published: (2025)
by: Katherine Forrester, et al.
Published: (2025)
Mechanistic model of minute virus of mice elution behavior in anion exchange chromatography purification
by: Ryunosuke Kitamura, et al.
Published: (2024)
by: Ryunosuke Kitamura, et al.
Published: (2024)
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5
by: Wenjin Xi, et al.
Published: (2025)
by: Wenjin Xi, et al.
Published: (2025)
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture
by: Célia Sanchez, et al.
Published: (2025)
by: Célia Sanchez, et al.
Published: (2025)
Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions
by: Akshay S. Chaubal, et al.
Published: (2026)
by: Akshay S. Chaubal, et al.
Published: (2026)
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications
by: Lucas Henrique Junges, et al.
Published: (2024)
by: Lucas Henrique Junges, et al.
Published: (2024)
Biotechnological potential of yeast cell wall: An overview
by: Fanny Machado Jofre, et al.
Published: (2024)
by: Fanny Machado Jofre, et al.
Published: (2024)
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy
by: Joseph R. Egan, et al.
Published: (2025)
by: Joseph R. Egan, et al.
Published: (2025)
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting
by: Lina Chakrabarti, et al.
Published: (2024)
by: Lina Chakrabarti, et al.
Published: (2024)
Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived CHO cell line
by: Theodore W. Peters, et al.
Published: (2026)
by: Theodore W. Peters, et al.
Published: (2026)
Similar Items
-
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based gene therapy
by: Yixuan Ming, et al.
Published: (2025) -
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines
by: Yu‐Chieh Lin, et al.
Published: (2025) -
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth
by: Han‐Jung Kuo, et al.
Published: (2025) -
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells
by: Min Lu, et al.
Published: (2024) -
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines
by: Niklas Krämer, et al.
Published: (2025)