Altered nicotinamide adenine dinucleotide metabolism drives cartilage degeneration and osteoarthritis
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Wiley
2025
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| author | Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam |
| author_facet | Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam |
| collection | Wiley Open Access |
| contents | Altered nicotinamide adenine dinucleotide metabolism drives cartilage degeneration and osteoarthritis Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam Clinical and Translational Medicine Abstract Background We previously conducted a comprehensive survey of energy metabolism in osteoarthritis (OA), revealing significant reductions of nicotinamide adenine dinucleotide (NAD + ) levels in OA cartilage. This study aimed to test whether NAD + deficiency present in OA plays a mechanistic role in disease development. Methods We conducted integrative analyses across human, murine, and rat OA models to examine NAD⁺ metabolism and its regulatory enzymes. The impact of pharmacological NAD⁺ augmentation (via nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR)) and genetic overexpression of the NAD⁺ biosynthetic enzyme NMN adenosyltransferase (NMNAT1) was tested in surgical and aging‐related OA models. Expression and function of the NAD⁺‐consuming enzyme poly (ADP‐ribose) polymerase 14 (PARP14) were examined via siRNA knockdown in chondrocytes under inflammatory conditions, coupled with metabolic assays and extracellular matrix gene profiling. Results NAD + levels were decreased in human and murine OA, accompanied by upregulation of both the NAD + biosynthetic enzyme Nicotinamide phosphoribosyltransferase (NAMPT) and the NAD + consuming enzyme PARP14. While NAMPT expression was elevated, its effect on total NAD⁺ may be offset by increased NAD⁺ consumption or substrate limitation under inflammatory conditions. Treatment with NAD + precursors and transgenic overexpression of NMNAT1 suppressed cartilage disruption during in aging murine and surgical rat model of OA. Increased expression of PARP14 in OA cartilage contributed to NAD + decline and promoted cartilage degeneration. Conclusions This study reveals that dysregulated NAD⁺ metabolism, driven by increased PARP14 consumption, constitutes a potential mechanism underlying OA pathogenesis. Our findings support the concept that enhancing NAD⁺ availability via precursors or biosynthetic pathway modulation may offer disease‐modifying effects at the molecular and histological level. Further investigation is needed to determine the functional and translational implications of targeting this pathway. Key points PARP14 is upregulated in OA cartilage and contributes to NAD⁺ depletion. PARP14 silencing restores NAD⁺ levels and represses OA‐related metabolic and matrix‐degrading changes. NAD⁺ precursor treatment and NMNAT1 overexpression protect against cartilage degeneration in aging and post‐traumatic OA models. 10.1002/ctm2.70513 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1002/ctm2.70513 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_ctm2_70513 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Altered nicotinamide adenine dinucleotide metabolism drives cartilage degeneration and osteoarthritis Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam Clinical and Translational Medicine Altered nicotinamide adenine dinucleotide metabolism drives cartilage degeneration and osteoarthritis Xiaoxin Wu Xiwei Fan Manuel Plan Terra Stark Tim McCubbin Roberto A. Barrero Maria Marinova Michael J. Bertoldo Dale M. Goss Lindsay E. Wu Ross Crawford Xinzhan Mao Indira Prasadam Clinical and Translational Medicine Abstract Background We previously conducted a comprehensive survey of energy metabolism in osteoarthritis (OA), revealing significant reductions of nicotinamide adenine dinucleotide (NAD + ) levels in OA cartilage. This study aimed to test whether NAD + deficiency present in OA plays a mechanistic role in disease development. Methods We conducted integrative analyses across human, murine, and rat OA models to examine NAD⁺ metabolism and its regulatory enzymes. The impact of pharmacological NAD⁺ augmentation (via nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR)) and genetic overexpression of the NAD⁺ biosynthetic enzyme NMN adenosyltransferase (NMNAT1) was tested in surgical and aging‐related OA models. Expression and function of the NAD⁺‐consuming enzyme poly (ADP‐ribose) polymerase 14 (PARP14) were examined via siRNA knockdown in chondrocytes under inflammatory conditions, coupled with metabolic assays and extracellular matrix gene profiling. Results NAD + levels were decreased in human and murine OA, accompanied by upregulation of both the NAD + biosynthetic enzyme Nicotinamide phosphoribosyltransferase (NAMPT) and the NAD + consuming enzyme PARP14. While NAMPT expression was elevated, its effect on total NAD⁺ may be offset by increased NAD⁺ consumption or substrate limitation under inflammatory conditions. Treatment with NAD + precursors and transgenic overexpression of NMNAT1 suppressed cartilage disruption during in aging murine and surgical rat model of OA. Increased expression of PARP14 in OA cartilage contributed to NAD + decline and promoted cartilage degeneration. Conclusions This study reveals that dysregulated NAD⁺ metabolism, driven by increased PARP14 consumption, constitutes a potential mechanism underlying OA pathogenesis. Our findings support the concept that enhancing NAD⁺ availability via precursors or biosynthetic pathway modulation may offer disease‐modifying effects at the molecular and histological level. Further investigation is needed to determine the functional and translational implications of targeting this pathway. Key points PARP14 is upregulated in OA cartilage and contributes to NAD⁺ depletion. PARP14 silencing restores NAD⁺ levels and represses OA‐related metabolic and matrix‐degrading changes. NAD⁺ precursor treatment and NMNAT1 overexpression protect against cartilage degeneration in aging and post‐traumatic OA models. 10.1002/ctm2.70513 http://creativecommons.org/licenses/by/4.0/ |
| title | Altered nicotinamide adenine dinucleotide metabolism drives cartilage degeneration and osteoarthritis |
| topic | Clinical and Translational Medicine |
| url | https://onlinelibrary.wiley.com/doi/10.1002/ctm2.70513 |