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| Main Authors: | , , , , , , , |
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| Format: | Artículo Open Access |
| Published: |
Wiley
2025
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| Subjects: | |
| Online Access: | https://iadns.onlinelibrary.wiley.com/doi/10.1002/efd2.70113 |
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Table of Contents:
- Innovation in Green Extraction of Essential Oils From Medicinal Aromatic Plants and Their Nano‐Formulation With Potential Anti‐Respiratory Viruses Activities Sobhy Mohamed Mohsen Mahmoud Mohamed Bahgat Azza A. Amin Amr M. Helal Dina Nadeem Abd‐Elshafy Sayed Saad Smuda Mohammad Ali Hesarinejad Tarek Gamal Abedelmaksoud eFood ABSTRACT Four methods were applied to extract essential oils (EOs) from dried peppermint, eucalyptus, thyme, and lemongrass. The highest EOs yield was obtained using steam distillation at the industrial scale and enzyme‐assisted extraction combined with ultrasonication at the laboratory scale. The bioactive constituents of the respective EOs were identified using gas chromatography–mass spectrometry (GC‐MS) as follows: peppermint (Isomenthone 16.03%, Menthol 35.40%, and Menthyl acetate 12.75%), thyme (p‐Cymene 15.37% and Thymol 66.70%), eucalyptus (p‐Cymene 9.18%, Eucalyptol 40.45%, and Spathulenol 12.74%), and lemongrass (Geraniol 15.85%, Geranial 29.78%, and Citral 22.78%). The four prepared EOs were combined at a fixed ratio (1:1:1:1) and nano‐formulated to obtain a 1% EOs preparation. The individual extracts as well as their nano‐mixture were evaluated for anti‐influenza A virus activity. Results demonstrated antiviral potential for all four extracts, while the nano‐mixture exhibited the strongest inhibitory effect. Moreover, the mixture showed a CC₅₀ value of 25.92 µL/ث on Vero E6 cells and an IC₅₀ value of 2.52 µL/mL against SARS‐CoV‐2 propagated in the same cell line. The selectivity index (SI = CC₅₀/IC₅₀) of the formulation was 10.28, which falls within the accepted threshold (SI ≥ 10) for bioactive compounds, suggesting its potential as an effective natural supplement against respiratory viruses. 10.1002/efd2.70113 http://creativecommons.org/licenses/by/4.0/