Routine Monitoring of CAR‐T‐Cells Expansion and Persistence in Patients With Aggressive Large B‐Cell Lymphoma by Flow Cytometry: A Single‐Center Experience
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Wiley
2025
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| author | Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus |
| author_facet | Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus |
| collection | Wiley Open Access |
| contents | Routine Monitoring of CAR‐T‐Cells Expansion and Persistence in Patients With Aggressive Large B‐Cell Lymphoma by Flow Cytometry: A Single‐Center Experience Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus Hematological Oncology ABSTRACT This retrospective, single‐center study evaluated routine flow cytometry (FC) monitoring of CAR‐T‐cells in 45 patients with aggressive large B‐cell lymphoma (LBCL). CAR‐T‐cells expansion was assessed from Day 0 to Month 12. Peak expansion occurred on Day 7 after axi‐cel (median 39.3% of total lymphocytes; 87 cells/mm 3 ) and Day 10 for tisa‐cel (median 8.1%; 33.2 cells/mm 3 ). Greater CAR‐T‐cells expansion was associated with immune‐related toxicity, with median peak levels reaching 52.8% in patients with grade 2 cytokine release syndrome (CRS) versus 6.6% in those without ( p < 0.001) and 60.8% in patients with immune effector cell‐associated neurotoxicity syndrome (ICANS) versus 15.3% in those without ( p < 0.001). Prolonged cytopenia was observed in 65% of patients and more frequently among those with greater CAR‐T‐cells expansion. This study highlights FC as a practical tool for monitoring CAR‐T‐cells dynamics in routine practice, with potential implications for early toxicity risk assessment and personalized supportive care. 10.1002/hon.70139 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| doi_str_mv | 10.1002/hon.70139 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_hon_70139 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Routine Monitoring of CAR‐T‐Cells Expansion and Persistence in Patients With Aggressive Large B‐Cell Lymphoma by Flow Cytometry: A Single‐Center Experience Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus Hematological Oncology Routine Monitoring of CAR‐T‐Cells Expansion and Persistence in Patients With Aggressive Large B‐Cell Lymphoma by Flow Cytometry: A Single‐Center Experience Alexandra Zduniak Jérémie Martinet Emilie Lévêque Stéphanie Becker David Tonnelet Elodie Dos Santos Claire Leroy Mustafa Alani Jean Rouvet Marine Brousseau Camille Giverne Alexis Cuffel Serge Jacquot Arnaud Roucheux Alice Veuiller Nicolas Lecornu Misa Eugene Norbert Olivier Boyer Hervé Tilly Fabrice Jardin Jean‐Baptiste Latouche Vincent Camus Hematological Oncology ABSTRACT This retrospective, single‐center study evaluated routine flow cytometry (FC) monitoring of CAR‐T‐cells in 45 patients with aggressive large B‐cell lymphoma (LBCL). CAR‐T‐cells expansion was assessed from Day 0 to Month 12. Peak expansion occurred on Day 7 after axi‐cel (median 39.3% of total lymphocytes; 87 cells/mm 3 ) and Day 10 for tisa‐cel (median 8.1%; 33.2 cells/mm 3 ). Greater CAR‐T‐cells expansion was associated with immune‐related toxicity, with median peak levels reaching 52.8% in patients with grade 2 cytokine release syndrome (CRS) versus 6.6% in those without ( p < 0.001) and 60.8% in patients with immune effector cell‐associated neurotoxicity syndrome (ICANS) versus 15.3% in those without ( p < 0.001). Prolonged cytopenia was observed in 65% of patients and more frequently among those with greater CAR‐T‐cells expansion. This study highlights FC as a practical tool for monitoring CAR‐T‐cells dynamics in routine practice, with potential implications for early toxicity risk assessment and personalized supportive care. 10.1002/hon.70139 http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| title | Routine Monitoring of CAR‐T‐Cells Expansion and Persistence in Patients With Aggressive Large B‐Cell Lymphoma by Flow Cytometry: A Single‐Center Experience |
| topic | Hematological Oncology |
| url | https://onlinelibrary.wiley.com/doi/10.1002/hon.70139 |