Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition
Fuente:
Wiley Open Access
Salvato in:
| Autori principali: | , , , , , , , |
|---|---|
| Natura: | Artículo Open Access |
| Pubblicazione: |
Wiley
2024
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1867012790008414208 |
|---|---|
| author | Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng |
| author_facet | Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng |
| collection | Wiley Open Access |
| contents | Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng Journal of the Science of Food and Agriculture AbstractBACKGROUNDIn response to growing concerns regarding heavy metal contamination in food, particularly chromium (Cr)(VI) contamination, this study presented a simple, sensitive and practical method for Cr(VI) detection.RESULTSA magnetic separation‐based capture–exponential enrichment ligand system evolution (SELEX) method was used to identify and characterize DNA aptamers with a high affinity for Cr(VI). An aptamer, Cr‐15, with a dissociation constant (Kd) of 4.42 ± 0.44 μmol L−1 was obtained after only eight rounds of selection. Further innovative methods combining molecular docking, dynamic simulation and thermodynamic analysis revealed that CrO42− could bind to the 19th and 20th guanine bases of Cr‐15 via hydrogen bonds. Crucially, a label‐free fluorometric aptasensor based on SYBR Green I was successfully constructed to detect CrO42−, achieving a linear detection range of 60–300 nmol L−1 with a lower limit of detection of 44.31 nmol L−1. Additionally, this aptasensor was able to quantitatively detect CrO42− in grapes and broccoli within 40 min, with spike recovery rates ranging from 89.22% to 108.05%. The designed fluorometric aptasensor exhibited high selectivity and could detect CrO42− in real samples without sample processing or target pre‐enrichment.CONCLUSIONThe aptasensor demonstrated its potential as a reliable tool for monitoring Cr(VI) contamination in fruit and vegetable products. © 2024 Society of Chemical Industry. 10.1002/jsfa.13631 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/jsfa.13631 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_jsfa_13631 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng Journal of the Science of Food and Agriculture Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition Jiangxiong Zhu Hao Yin Sisi Zheng Hong Yu Linnan Yang Lumei Wang Xueqing Geng Yun Deng Journal of the Science of Food and Agriculture AbstractBACKGROUNDIn response to growing concerns regarding heavy metal contamination in food, particularly chromium (Cr)(VI) contamination, this study presented a simple, sensitive and practical method for Cr(VI) detection.RESULTSA magnetic separation‐based capture–exponential enrichment ligand system evolution (SELEX) method was used to identify and characterize DNA aptamers with a high affinity for Cr(VI). An aptamer, Cr‐15, with a dissociation constant (Kd) of 4.42 ± 0.44 μmol L−1 was obtained after only eight rounds of selection. Further innovative methods combining molecular docking, dynamic simulation and thermodynamic analysis revealed that CrO42− could bind to the 19th and 20th guanine bases of Cr‐15 via hydrogen bonds. Crucially, a label‐free fluorometric aptasensor based on SYBR Green I was successfully constructed to detect CrO42−, achieving a linear detection range of 60–300 nmol L−1 with a lower limit of detection of 44.31 nmol L−1. Additionally, this aptasensor was able to quantitatively detect CrO42− in grapes and broccoli within 40 min, with spike recovery rates ranging from 89.22% to 108.05%. The designed fluorometric aptasensor exhibited high selectivity and could detect CrO42− in real samples without sample processing or target pre‐enrichment.CONCLUSIONThe aptasensor demonstrated its potential as a reliable tool for monitoring Cr(VI) contamination in fruit and vegetable products. © 2024 Society of Chemical Industry. 10.1002/jsfa.13631 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Tag‐free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition |
| topic | Journal of the Science of Food and Agriculture |
| url | https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jsfa.13631 |