Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial–mesenchymal transition, and metastasis in breast cancer
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2025
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| author | Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant |
| author_facet | Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant |
| collection | Wiley Open Access |
| contents | Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial–mesenchymal transition, and metastasis in breast cancer Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant Journal of the Science of Food and Agriculture Abstract BACKGROUND Breast cancer (BC) is a life‐threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant Garcinia cowa Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through in silico , in vitro , and in vivo studies using a mouse model of BC. RESULTS The results revealed that chamuangone (10 mg L −1 ) inhibited cancer cell proliferation by inducing G0/G1 phase arrest and apoptosis in 4T1‐luciferase (4T1‐luc) and Michigan Cancer Foundation‐7 (MCF‐7) cells. It also modulated the levels of key apoptotic markers, including B‐cell lymphoma 2 (Bcl‐2), Bcl‐2‐associated X protein (Bax), and caspase‐3. Similarly, in vivo studies demonstrated that chamuangone significantly ( P < 0.05) inhibited breast tumor growth and lung metastasis at a dose of 100 mg kg⁻¹ body weight, in comparison with the standard drug doxorubicin (5 mg kg⁻¹ body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM‐associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki‐67 antigen (Ki‐67), cluster of differentiation 44 (CD44), E‐cadherin, N‐cadherin, vascular endothelial growth factor A (VEGFA), interleukin‐6 (IL‐6), and tumor necrosis factor‐ α (TNF‐ α ), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial–mesenchymal transition (EMT), cancer cell proliferation, and metastasis. In silico studies also demonstrated strong interactions between chamuangone and BCLM‐related targets, along with encouraging pharmacokinetic and toxicokinetic profiles. CONCLUSION This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. © 2025 Society of Chemical Industry. 10.1002/jsfa.70175 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/jsfa.70175 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_jsfa_70175 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial–mesenchymal transition, and metastasis in breast cancer Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant Journal of the Science of Food and Agriculture Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial–mesenchymal transition, and metastasis in breast cancer Asad Ur Rahman Naveed Ullah Khan Abdul Basit Muhammad Khan Jiang Ni Pharkphoom Panichayupakaranant Journal of the Science of Food and Agriculture Abstract BACKGROUND Breast cancer (BC) is a life‐threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant Garcinia cowa Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through in silico , in vitro , and in vivo studies using a mouse model of BC. RESULTS The results revealed that chamuangone (10 mg L −1 ) inhibited cancer cell proliferation by inducing G0/G1 phase arrest and apoptosis in 4T1‐luciferase (4T1‐luc) and Michigan Cancer Foundation‐7 (MCF‐7) cells. It also modulated the levels of key apoptotic markers, including B‐cell lymphoma 2 (Bcl‐2), Bcl‐2‐associated X protein (Bax), and caspase‐3. Similarly, in vivo studies demonstrated that chamuangone significantly ( P < 0.05) inhibited breast tumor growth and lung metastasis at a dose of 100 mg kg⁻¹ body weight, in comparison with the standard drug doxorubicin (5 mg kg⁻¹ body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM‐associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki‐67 antigen (Ki‐67), cluster of differentiation 44 (CD44), E‐cadherin, N‐cadherin, vascular endothelial growth factor A (VEGFA), interleukin‐6 (IL‐6), and tumor necrosis factor‐ α (TNF‐ α ), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial–mesenchymal transition (EMT), cancer cell proliferation, and metastasis. In silico studies also demonstrated strong interactions between chamuangone and BCLM‐related targets, along with encouraging pharmacokinetic and toxicokinetic profiles. CONCLUSION This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. © 2025 Society of Chemical Industry. 10.1002/jsfa.70175 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial–mesenchymal transition, and metastasis in breast cancer |
| topic | Journal of the Science of Food and Agriculture |
| url | https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jsfa.70175 |