| _version_ | 1867019370546331648 |
|---|---|
| author | Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel |
| author_facet | Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel |
| collection | Wiley Open Access |
| contents | A Comprehensive Flow Cytometry Panel for Analysis of Idiopathic Subglottic Stenosis Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel Otolaryngology–Head and Neck Surgery AbstractObjectiveTo present a comprehensive flow cytometry panel for idiopathic subglottic stenosis (iSGS).Study DesignControlled ex vivo cohort study.SettingTertiary care academic hospital in a metropolitan area.MethodsFlow cytometry and single‐cell RNA sequencing were performed on 9 paired normal and scar tissue samples from iSGS patients. Flow cytometry was used to assess the presence of myeloid (CD11b, CD14, CD15, Siglec8), lymphoid (CD3, CD4, CD8, gamma delta [γδ], FOXP3), endothelial (CD31), fibroblast (CD90, SMA), and epithelial (CD326, CK5) markers.ResultsOn flow cytometry, iSGS scar is characterized by an increased presence of myeloid, lymphoid, endothelial, and fibroblast cell types, but a decreased presence of epithelial cells. In the myeloid lineage, iSGS scar samples demonstrated increased CD11b+ monocytes (P < .001), Siglec8+ eosinophils (P = .03), and CD14+ monocytes (P = .02). In the lymphoid lineage, iSGS scar demonstrated increased CD3+ T‐cells (P < .001), CD4+ helper T‐cells (P < .001), γδ+ T‐cells (P < .001), and FOXP3+ regulatory T‐cells (P = .002). iSGS scar exhibited specific increases in CD90+ (P = .04) and SMA+ (P < .001) fibroblasts but decreased CD326+ (E‐cadherin) epithelial cells (P = .01) relative to normal samples.ConclusionWe present a comprehensive flow cytometry panel for iSGS. This flow panel may serve as a common platform among airway scientists to elucidate the cellular mechanisms underpinning iSGS and other upper airway pathologies. Scar iSGS samples demonstrate a distinct cellular profile relative to normal iSGS specimens, exhibiting increased fibroblast, endothelial, and inflammatory cell types but decreased epithelium. 10.1002/ohn.754 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/ohn.754 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_ohn_754 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | A Comprehensive Flow Cytometry Panel for Analysis of Idiopathic Subglottic Stenosis Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel Otolaryngology–Head and Neck Surgery A Comprehensive Flow Cytometry Panel for Analysis of Idiopathic Subglottic Stenosis Raymond J. So Samuel L. Collins Yee Chan‐Li Ioan Lina Alexander Gelbard Kevin M. Motz Alexander T. Hillel Otolaryngology–Head and Neck Surgery AbstractObjectiveTo present a comprehensive flow cytometry panel for idiopathic subglottic stenosis (iSGS).Study DesignControlled ex vivo cohort study.SettingTertiary care academic hospital in a metropolitan area.MethodsFlow cytometry and single‐cell RNA sequencing were performed on 9 paired normal and scar tissue samples from iSGS patients. Flow cytometry was used to assess the presence of myeloid (CD11b, CD14, CD15, Siglec8), lymphoid (CD3, CD4, CD8, gamma delta [γδ], FOXP3), endothelial (CD31), fibroblast (CD90, SMA), and epithelial (CD326, CK5) markers.ResultsOn flow cytometry, iSGS scar is characterized by an increased presence of myeloid, lymphoid, endothelial, and fibroblast cell types, but a decreased presence of epithelial cells. In the myeloid lineage, iSGS scar samples demonstrated increased CD11b+ monocytes (P < .001), Siglec8+ eosinophils (P = .03), and CD14+ monocytes (P = .02). In the lymphoid lineage, iSGS scar demonstrated increased CD3+ T‐cells (P < .001), CD4+ helper T‐cells (P < .001), γδ+ T‐cells (P < .001), and FOXP3+ regulatory T‐cells (P = .002). iSGS scar exhibited specific increases in CD90+ (P = .04) and SMA+ (P < .001) fibroblasts but decreased CD326+ (E‐cadherin) epithelial cells (P = .01) relative to normal samples.ConclusionWe present a comprehensive flow cytometry panel for iSGS. This flow panel may serve as a common platform among airway scientists to elucidate the cellular mechanisms underpinning iSGS and other upper airway pathologies. Scar iSGS samples demonstrate a distinct cellular profile relative to normal iSGS specimens, exhibiting increased fibroblast, endothelial, and inflammatory cell types but decreased epithelium. 10.1002/ohn.754 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | A Comprehensive Flow Cytometry Panel for Analysis of Idiopathic Subglottic Stenosis |
| topic | Otolaryngology–Head and Neck Surgery |
| url | https://aao-hnsfjournals.onlinelibrary.wiley.com/doi/10.1002/ohn.754 |