Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Artículo Open Access |
| Published: |
Wiley
2025
|
| Subjects: | |
| Online Access: | https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6437 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867016347575123968 |
|---|---|
| author | Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini |
| author_facet | Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini |
| collection | Wiley Open Access |
| contents | Mucinous cystic neoplasms and simple mucinous cysts are two distinct precursors of pancreatic cancer: clinicopathological, genomic, and transcriptomic characterization Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini The Journal of Pathology AbstractMucinous cystic neoplasms (MCNs) of the pancreas are macroscopic precursors of pancreatic cancer. A similar cystic lesion but lacking the ovarian‐type subepithelial stroma has been recently defined as a simple mucinous cyst (SMC); however, its nature remains unclear. This study aims to define the clinicopathological and molecular profiles of a cohort of MCNs and SMCs of the pancreas and their associated invasive carcinoma. Overall, 23 cases were identified, comprising 19 MCNs and 4 SMCs with co‐occurring invasive carcinoma. A multiregional (two samples from each cystic lesion and one from the adenocarcinoma) DNA and RNA sequencing approach was used. The key findings can be summarized as follows: (1) Molecular association: In 22/23 cases (95.7%), the concomitant mucinous cyst and invasive carcinoma shared specific genomic alterations, establishing for the first time that SMC is a true precursor of pancreatic cancer. (2) Clinical behavior: carcinomas arising from SMC appeared to be more aggressive than those arising from MCN. (3) Mutational profile: both cyst types showed significant similarities to conventional pancreatic ductal adenocarcinoma (PDAC), with KRAS and TP53 the most commonly altered genes. (4) Intracystic heterogeneity: while most molecular alterations were present in both analyzed cystic areas, RNF43 showed the highest heterogeneity. (5) CDKN2A: its alterations were predominantly restricted to the invasive component, suggesting a role in driving the invasion in a subset of cases. CNKN2A may also serve as a potential biomarker for identifying high‐risk cysts. (6) RNAseq: most cases showed a switch from the classical to the basal transcriptome subtype during the progression from cystic neoplasms to invasive cancers. These findings establish SMCs as new precursors of pancreatic cancer and provide critical insights into the tumorigenesis of MCNs, with potential immediate implications for tumor taxonomy and clinical management. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. 10.1002/path.6437 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1002/path.6437 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_path_6437 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2025 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Mucinous cystic neoplasms and simple mucinous cysts are two distinct precursors of pancreatic cancer: clinicopathological, genomic, and transcriptomic characterization Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini The Journal of Pathology Mucinous cystic neoplasms and simple mucinous cysts are two distinct precursors of pancreatic cancer: clinicopathological, genomic, and transcriptomic characterization Antonio Pea Michele Bevere Anastasios Gkountakos Davide Pasini Denise Fiorini Andrea Mafficini Stela Golovco Michele Simbolo Serena Pedron Concetta Sciammarella Paola Mattiolo Aldo Mombello Manuela Villanova Carlotta Franzina Francesca Masetto Calogero Ciulla Nicola Sperandio Kohei Fujikura Masha S. Ahadi Jaswinder S. Samra Amber L. Johns Joanne Verheij Martijn W.J. Stommel Hjalmar van Santvoort Leonor Schubert Santana Giuseppe Malleo Michele Milella Lodewijk A. A. Brosens Laura D. Wood David K. Chang Riccardo De Robertis Mirko D'Onofrio Anthony J. Gill Roberto Salvia Vincenzo Corbo Rita T. Lawlor Aldo Scarpa Claudio Luchini The Journal of Pathology AbstractMucinous cystic neoplasms (MCNs) of the pancreas are macroscopic precursors of pancreatic cancer. A similar cystic lesion but lacking the ovarian‐type subepithelial stroma has been recently defined as a simple mucinous cyst (SMC); however, its nature remains unclear. This study aims to define the clinicopathological and molecular profiles of a cohort of MCNs and SMCs of the pancreas and their associated invasive carcinoma. Overall, 23 cases were identified, comprising 19 MCNs and 4 SMCs with co‐occurring invasive carcinoma. A multiregional (two samples from each cystic lesion and one from the adenocarcinoma) DNA and RNA sequencing approach was used. The key findings can be summarized as follows: (1) Molecular association: In 22/23 cases (95.7%), the concomitant mucinous cyst and invasive carcinoma shared specific genomic alterations, establishing for the first time that SMC is a true precursor of pancreatic cancer. (2) Clinical behavior: carcinomas arising from SMC appeared to be more aggressive than those arising from MCN. (3) Mutational profile: both cyst types showed significant similarities to conventional pancreatic ductal adenocarcinoma (PDAC), with KRAS and TP53 the most commonly altered genes. (4) Intracystic heterogeneity: while most molecular alterations were present in both analyzed cystic areas, RNF43 showed the highest heterogeneity. (5) CDKN2A: its alterations were predominantly restricted to the invasive component, suggesting a role in driving the invasion in a subset of cases. CNKN2A may also serve as a potential biomarker for identifying high‐risk cysts. (6) RNAseq: most cases showed a switch from the classical to the basal transcriptome subtype during the progression from cystic neoplasms to invasive cancers. These findings establish SMCs as new precursors of pancreatic cancer and provide critical insights into the tumorigenesis of MCNs, with potential immediate implications for tumor taxonomy and clinical management. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. 10.1002/path.6437 http://creativecommons.org/licenses/by/4.0/ |
| title | Mucinous cystic neoplasms and simple mucinous cysts are two distinct precursors of pancreatic cancer: clinicopathological, genomic, and transcriptomic characterization |
| topic | The Journal of Pathology |
| url | https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6437 |