Buchumschlag

Enhanced protein extraction and quantification protocol for microsamples: An ultra‐sensitive workflow for low‐volume, low‐concentration total protein lysates

Fuente: Wiley Open Access

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Taylor Wilcox, Michael E. Widlansky, Justin Westhoff, Jingli Wang, Rong Ying, Abigail Thorgerson, Michelle L. Roberts
Format:	Artículo Open Access
Veröffentlicht:	Wiley 2025
Schlagworte:	Protein Science
Online-Zugang:	Acceder al recurso
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge

Screening de novo designed protein binders in unpurified lysate using flow induced dispersion analysis
von: Francisca Pinheiro, et al.
Veröffentlicht: (2025)

How sensitive are protein hydration shells to electrolyte concentration and protein composition?
von: H. Geraili Daronkola, et al.
Veröffentlicht: (2024)

Predicting protein-protein interaction with interpretable bilinear attention network.
von: Han, Yong, et al.
Veröffentlicht: (2025)

Xplor‐NIH: Better parameters and protocols for NMR protein structure determination
von: Guillermo A. Bermejo, et al.
Veröffentlicht: (2024)

Small heat shock proteins HspB1 and HspB5 differentially alter the condensation and aggregation of the TDP ‐43 low‐complexity domain
von: Thomas B. Walker, et al.
Veröffentlicht: (2026)

Solid‐state NMR protocols for unveiling dynamics and (drug) interactions of membrane‐bound proteins
von: Alessia Lasorsa, et al.
Veröffentlicht: (2025)

Glycopolymers stabilize protein folding and protein–protein interactions via enthalpic interactions
von: Sabrina M. Richter, et al.
Veröffentlicht: (2025)

Fig. 4. Thermal hysteresis vs. recombinant Antifreeze protein (AFP) concentration in buffers of salinity
von: Uhlig, Christiane, et al.
Veröffentlicht: (2011)

pLM ‐Repeat: Exploiting the sequence representations of protein language models for sensitive repeat detection
von: Kaiyu Qiu, et al.
Veröffentlicht: (2026)

Bromodomain-containing proteins in the unicellular eukaryote .
von: Zhang, Zhe, et al.
Veröffentlicht: (2025)

Comparative analysis of the extent of protein–protein interactions in icosahedral viral capsids
von: Noah J. Zimmerman, et al.
Veröffentlicht: (2025)

A high‐sensitivity stopped‐flow EPR system to monitor millisecond conformational kinetics in spin‐labeled proteins
von: Alexander M. Garces, et al.
Veröffentlicht: (2025)

Multifactorial interaction and influence of culture conditions on yellow fluorescent protein production in Phaeodactylum tricornutum.
von: Augustine, Arun, et al.
Veröffentlicht: (2025)

Theta‐curves in proteins
von: Pawel Dabrowski‐Tumanski, et al.
Veröffentlicht: (2024)

A significance score for protein–protein interaction models through random docking
von: Katherine Maia McCoy, et al.
Veröffentlicht: (2024)

Predicting compatibility between ferredoxins and the Fe protein of nitrogenase using in silico protein modeling
von: Adity Biswas, et al.
Veröffentlicht: (2026)

Inhibitory protein–protein interactions of the SIRT1 deacetylase are choreographed by post‐translational modification
von: Troy C. Krzysiak, et al.
Veröffentlicht: (2024)

Allosteric regulation of the tyrosine phosphatase PTP1B by a protein–protein interaction
von: Cassandra A. Chartier, et al.
Veröffentlicht: (2024)

The importance of input sequence set to consensus‐derived proteins and their relationship to reconstructed ancestral proteins
von: Charlotte Nixon, et al.
Veröffentlicht: (2024)

Discovery of periplasmic solute binding proteins with specificity for ketone bodies: β‐hydroxybutyrate binding proteins
von: Bryant J. Kane, et al.
Veröffentlicht: (2024)

Amino acid sequence encodes protein abundance shaped by protein stability at reduced synthesis cost
von: Filip Buric, et al.
Veröffentlicht: (2024)

AggNet: Advancing protein aggregation analysis through deep learning and protein language model
von: Wenjia He, et al.
Veröffentlicht: (2025)

Cell surface β‐lactamase recruitment: A facile selection to identify protein–protein interactions
von: Jordan A. Hinmon, et al.
Veröffentlicht: (2024)

Increasing the fraction of correct solutions in ensembles of protein–protein docking models by an iterative consensus algorithm
von: Tiziana Ricciardelli, et al.
Veröffentlicht: (2025)

Engineered cyclodextrin-protein domain interface modulation and application in nanoscale delivery systems development for therapeutic proteins.
von: Wang, Qiyue, et al.
Veröffentlicht: (2025)

ProTaxoVis-protein taxonomic visualisation of presence.
von: Hsieh, Yin-Chen, et al.
Veröffentlicht: (2025)

Partition of protein from sea urchin: Diadema setosum in different salts
von: Omollo, S.O.
Veröffentlicht: ()

Soy protein processing impacts the ice recrystallization inhibition activity of protein hydrolysates
von: Madison Fomich, et al.
Veröffentlicht: (2024)

Chemical and temporal manipulation of early steps in protein assembly tunes the structure and intermolecular interactions of protein‐based materials
von: Valeria Italia, et al.
Veröffentlicht: (2025)

MemPPI platform for measuring and engineering membrane protein–protein interactions in mammalian cells via split nanoluciferase
von: Weiyi Tang, et al.
Veröffentlicht: (2026)

KiNext: a portable and scalable workflow for the identification and classification of protein kinases.
von: Hellec, Elisabeth, et al.
Veröffentlicht: (2024)

Efficient and economic protein labeling for NMR in mammalian expression systems: Application to a preT‐cell and T‐cell receptor protein
von: Robert J. Mallis, et al.
Veröffentlicht: (2024)

Calpain– MMP 3 activation restricts nuclear uptake during protein transduction domain TAT ‐mediated protein delivery
von: Jin Hyeop Kim, et al.
Veröffentlicht: (2026)

Investigation of the structure of protein–polymer conjugates in solution reveals the impact of protein deuteration and the size of the polymer on its thermal stability
von: Daniela Russo, et al.
Veröffentlicht: (2024)

A conserved motif in Henipavirus P/V/W proteins drives the fibrillation of the W protein from Hendra virus
von: Frank Gondelaud, et al.
Veröffentlicht: (2025)

PackPPI: An integrated framework for protein–protein complex side‐chain packing and ΔΔG prediction based on diffusion model
von: Jingkai Zhang, et al.
Veröffentlicht: (2025)

Engineering G protein‐coupled receptors for stabilization
von: João Paulo L. Velloso, et al.
Veröffentlicht: (2024)

Dynamic disorder is crucial for mitochondrial protein import
von: Jakob Schneider, et al.
Veröffentlicht: (2026)

Classifying protein kinase conformations with machine learning
von: Ivan Reveguk, et al.
Veröffentlicht: (2024)

Design of proteins by parallel tempering in the sequence space
von: Preet Kalani, et al.
Veröffentlicht: (2025)