Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein
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| Formato: | Artículo Open Access |
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Wiley
2024
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| _version_ | 1867018534692847616 |
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| author | Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof |
| author_facet | Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof |
| collection | Wiley Open Access |
| contents | Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof Journal of Peptide Science Bicyclic peptides are important chemical tools that can function, for example, as bioactive ligands switching on/off signaling pathways mediated by guanine nucleotide‐binding proteins as bicycles are more broadly applicable. Despite their relevance in medicinal chemistry, the synthesis of such peptides is challenging, and the final yield is highly dependent on the chemical composition and physicochemical properties of the scaffold. We recently discovered novel, state‐specific peptide modulators targeting the Gαi protein, namely, GPM‐2/GPM‐3, by screening a one‐bead‐two‐compound combinatorial library. A more detailed analysis, including sequence alignments and computer‐assisted conformational studies based on the hit compounds, revealed the new peptide 10 as a potential macrobicyclic Gαi ligand sharing high sequence similarity to the known Gαi modulators. The Gαs protein was included in this study for comparison and to unravel the criteria for the specificity of modulator binding to Gαi versus Gαs. This work provides in‐depth computer‐assisted experimental studies for the analysis of novel macrobicyclic, library‐derived Gαi protein ligands. The sequence and structural comparison of 10 with the lead compounds GPM‐2 and GPM‐3 reveals the importance of the size and amino acid composition of one ring of the bicyclic system and suggests features enhancing the binding affinity of the peptides to the Gαi protein. 10.1002/psc.3565 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1002/psc.3565 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_psc_3565 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof Journal of Peptide Science Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein Anna Pepanian F. Ayberk Binbay Dehua Pei Diana Imhof Journal of Peptide Science Bicyclic peptides are important chemical tools that can function, for example, as bioactive ligands switching on/off signaling pathways mediated by guanine nucleotide‐binding proteins as bicycles are more broadly applicable. Despite their relevance in medicinal chemistry, the synthesis of such peptides is challenging, and the final yield is highly dependent on the chemical composition and physicochemical properties of the scaffold. We recently discovered novel, state‐specific peptide modulators targeting the Gαi protein, namely, GPM‐2/GPM‐3, by screening a one‐bead‐two‐compound combinatorial library. A more detailed analysis, including sequence alignments and computer‐assisted conformational studies based on the hit compounds, revealed the new peptide 10 as a potential macrobicyclic Gαi ligand sharing high sequence similarity to the known Gαi modulators. The Gαs protein was included in this study for comparison and to unravel the criteria for the specificity of modulator binding to Gαi versus Gαs. This work provides in‐depth computer‐assisted experimental studies for the analysis of novel macrobicyclic, library‐derived Gαi protein ligands. The sequence and structural comparison of 10 with the lead compounds GPM‐2 and GPM‐3 reveals the importance of the size and amino acid composition of one ring of the bicyclic system and suggests features enhancing the binding affinity of the peptides to the Gαi protein. 10.1002/psc.3565 http://creativecommons.org/licenses/by/4.0/ |
| title | Design, synthesis, and analysis of macrobicyclic peptides for targeting the Gαi protein |
| topic | Journal of Peptide Science |
| url | https://onlinelibrary.wiley.com/doi/10.1002/psc.3565 |