Identification and Mechanistic Study of a Novel Keap1‐Targeting Antioxidant Peptide From Ulva prolifera Protein
Fuente:
Wiley Open Access
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo Open Access |
| Publicado: |
Wiley
2026
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1867017959783792640 |
|---|---|
| author | Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li |
| author_facet | Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li |
| collection | Wiley Open Access |
| contents | Identification and Mechanistic Study of a Novel Keap1‐Targeting Antioxidant Peptide From Ulva prolifera Protein Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li Journal of Peptide Science ABSTRACT Activating Nrf2 by targeting the Keap1‐Nrf2 signaling axis has emerged as a viable therapeutic approach for managing oxidative stress and its associated disorders. This study employed virtual digestion, ADMET profiling, and molecular docking to identify antioxidant peptides derived from Ulva prolifera protein. A novel tetrapeptide (WDGL) was ultimately discovered, demonstrating favorable aqueous solubility, non‐toxicity, high intestinal absorption efficiency, and blood–brain barrier permeability. WDGL established four H‐bond interactions with some key sites of Keap1 (Arg380, Arg415, Ile416, Leu365). Besides, WDGL reduces ABTS· + and ferric‐tripyridyltriazine (Fe 3+ ‐TPTZ) in vitro and promotes the expression of GSH‐Px while increasing GSH‐Px and SOD enzyme activity to eliminate ROS in LPS‐treated EA.hy926 cells. Furthermore, this peptide could reduce the content of ROS and ET‐1 in Ang II‐induced EA.hy926 cells. The result suggested that WDGL may alleviate EA.hy926 oxidative damage by activating the Keap1‐Nrf2 signaling pathway. 10.1002/psc.70100 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/psc.70100 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_psc_70100 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2026 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Identification and Mechanistic Study of a Novel Keap1‐Targeting Antioxidant Peptide From Ulva prolifera Protein Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li Journal of Peptide Science Identification and Mechanistic Study of a Novel Keap1‐Targeting Antioxidant Peptide From Ulva prolifera Protein Shaohui Zhu Shanglian Liu Huashan Yao Lan Fang Hongbo Xu Zhiyong Li Journal of Peptide Science ABSTRACT Activating Nrf2 by targeting the Keap1‐Nrf2 signaling axis has emerged as a viable therapeutic approach for managing oxidative stress and its associated disorders. This study employed virtual digestion, ADMET profiling, and molecular docking to identify antioxidant peptides derived from Ulva prolifera protein. A novel tetrapeptide (WDGL) was ultimately discovered, demonstrating favorable aqueous solubility, non‐toxicity, high intestinal absorption efficiency, and blood–brain barrier permeability. WDGL established four H‐bond interactions with some key sites of Keap1 (Arg380, Arg415, Ile416, Leu365). Besides, WDGL reduces ABTS· + and ferric‐tripyridyltriazine (Fe 3+ ‐TPTZ) in vitro and promotes the expression of GSH‐Px while increasing GSH‐Px and SOD enzyme activity to eliminate ROS in LPS‐treated EA.hy926 cells. Furthermore, this peptide could reduce the content of ROS and ET‐1 in Ang II‐induced EA.hy926 cells. The result suggested that WDGL may alleviate EA.hy926 oxidative damage by activating the Keap1‐Nrf2 signaling pathway. 10.1002/psc.70100 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Identification and Mechanistic Study of a Novel Keap1‐Targeting Antioxidant Peptide From Ulva prolifera Protein |
| topic | Journal of Peptide Science |
| url | https://onlinelibrary.wiley.com/doi/10.1002/psc.70100 |