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| Natura: | Artículo Open Access |
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Wiley
2024
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| Accesso online: | https://onlinelibrary.wiley.com/doi/10.1002/ptr.8167 |
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| author | Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao |
| author_facet | Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao |
| collection | Wiley Open Access |
| contents | Dihydrocapsaicin suppresses the STING‐mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia–reperfusion injury of perforator skin flap Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao Phytotherapy Research AbstractAvascular necrosis frequently occurs as a complication following surgery involving the distal perforator flap. Dihydrocapsaicin (DHC) can protect tissue from ischemia–reperfusion (I/R) injury, but its specific role in multizone perforator flaps remains unclear. In this study, the prospective target of DHC in the context of I/R injury was predicted using network pharmacology analysis. Flap viability was determined through survival area analysis, laser Doppler blood flow, angiograms, and histological examination. The expressions of angiogenesis, apoptosis, NLR family pyrin domain containing 3 (NLRP3) inflammasome, oxidative stress, and molecules related to cyclic guanosine monophosphate (GMP)‐adenosine monophosphate synthase (cGAS)‐interferon gene stimulant (STING) pathway were assessed using western blotting, immunofluorescence, TUNEL staining, and dihydroethidium (DHE) staining. Our finding revealed that DHC promoted the perforator flap survival, which involves the cGAS–STING pathway, oxidative stress, NLRP3 inflammasome, apoptosis, and angiogenesis. DHC induced oxidative stress resistance and suppressed the NLRP3 inflammasome, preventing apoptosis in vascular endothelial cells. Through regulation of STING pathway, DHC controlled oxidative stress in endothelial cells and NLRP3 levels in ischemic flaps. However, activation of the cGAS–STING pathway led to the accumulation of reactive oxygen species (ROS) and NLRP3 inflammasome, thereby diminishing the protective role of DHC. DHC enhanced the survival of multidomain perforator flaps by suppressing the cGAS–STING pathway, oxidative stress, and the formation of NLRP3 inflammasome. These findings unveil a potentially novel mechanism with clinical significance for promoting the survival of multidomain perforator flaps. 10.1002/ptr.8167 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| doi_str_mv | 10.1002/ptr.8167 |
| format | Artículo Open Access |
| id | wiley_oa_10_1002_ptr_8167 |
| institution | Wiley Open Access |
| license_str_mv | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Dihydrocapsaicin suppresses the STING‐mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia–reperfusion injury of perforator skin flap Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao Phytotherapy Research Dihydrocapsaicin suppresses the STING‐mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia–reperfusion injury of perforator skin flap Yingying Lai Ningning Yang Xuankuai Chen Xianhui Ma Zhuliu Chen Chengji Dong Gaoxiang Yu Yingying Huang Donghao Shi Pin Fang Kejian Fu Renhao Jiang Cong Mao Jian Ding Weiyang Gao Phytotherapy Research AbstractAvascular necrosis frequently occurs as a complication following surgery involving the distal perforator flap. Dihydrocapsaicin (DHC) can protect tissue from ischemia–reperfusion (I/R) injury, but its specific role in multizone perforator flaps remains unclear. In this study, the prospective target of DHC in the context of I/R injury was predicted using network pharmacology analysis. Flap viability was determined through survival area analysis, laser Doppler blood flow, angiograms, and histological examination. The expressions of angiogenesis, apoptosis, NLR family pyrin domain containing 3 (NLRP3) inflammasome, oxidative stress, and molecules related to cyclic guanosine monophosphate (GMP)‐adenosine monophosphate synthase (cGAS)‐interferon gene stimulant (STING) pathway were assessed using western blotting, immunofluorescence, TUNEL staining, and dihydroethidium (DHE) staining. Our finding revealed that DHC promoted the perforator flap survival, which involves the cGAS–STING pathway, oxidative stress, NLRP3 inflammasome, apoptosis, and angiogenesis. DHC induced oxidative stress resistance and suppressed the NLRP3 inflammasome, preventing apoptosis in vascular endothelial cells. Through regulation of STING pathway, DHC controlled oxidative stress in endothelial cells and NLRP3 levels in ischemic flaps. However, activation of the cGAS–STING pathway led to the accumulation of reactive oxygen species (ROS) and NLRP3 inflammasome, thereby diminishing the protective role of DHC. DHC enhanced the survival of multidomain perforator flaps by suppressing the cGAS–STING pathway, oxidative stress, and the formation of NLRP3 inflammasome. These findings unveil a potentially novel mechanism with clinical significance for promoting the survival of multidomain perforator flaps. 10.1002/ptr.8167 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| title | Dihydrocapsaicin suppresses the STING‐mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia–reperfusion injury of perforator skin flap |
| topic | Phytotherapy Research |
| url | https://onlinelibrary.wiley.com/doi/10.1002/ptr.8167 |