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| Formato: | Artículo Open Access |
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Wiley
2024
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| Acceso en línea: | https://onlinelibrary.wiley.com/doi/10.1111/cod.14711 |
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| author | Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle |
| author_facet | Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle |
| collection | Wiley Open Access |
| contents | A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle Contact Dermatitis AbstractBackgroundLimonene and linalool are used in cosmetic products for their floral scents, but their oxidation products are strong contact allergens whose mechanisms of action are still not fully understood.ObjectivesThe effects of limonene hydroperoxide (Lim‐2‐OOH) and linalool hydroperoxides (Lin‐6/7‐OOH) on the lipid profile of a human keratinocyte cell line (HaCaT) were evaluated. 2,4‐Dinitrofluorobenzene (DNFB) was also included.MethodsLim‐2‐OOH and Lin‐6/7‐OOH were synthesised according to previous methods. HaCaT cells were treated with allergens (10 μM) for 24 h and the cellular lipid extracts were analysed by C18 liquid chromatography with tandem mass spectrometry (LC–MS/MS). Data analysis was performed using Lipostar software. Statistical analysis was carried out using Metaboanalyst and R software.ResultsAll three sensitisers used caused significant changes in the lipidome of HaCaT cells in a similar trend. There was an upregulation in several plasmanyl/plasmenyl phospholipids (O‐/P‐phosphatidylcholines [PC] and O‐/P‐phosphatidylethanolamines [PE]), sphingolipids (HexCer) and triacylglycerol lipid species, and a decrease in some polyunsaturated fatty acids‐containing phospholipid (PE and PC) species suggesting oxidative stress and inflammation.ConclusionsThis study is the first to evaluate the plasticity of the HaCaT cell lipidome in response to allylic hydroperoxide allergens Lim‐2‐OOH and Lin‐6/7‐OOH, together with the experimental contact allergen DNFB. These allergens are able to upregulate and downregulate certain lipid classes to a varying degree. 10.1111/cod.14711 http://creativecommons.org/licenses/by-nc/4.0/ |
| doi_str_mv | 10.1111/cod.14711 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_cod_14711 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by-nc/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle Contact Dermatitis A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes Aaron I. Moore Ana S. P. Moreira Inês M. S. Guerra Laura Goracci Pedro Domingues Tânia Melo M. Rosário Domingues Niamh M. O'Boyle Contact Dermatitis AbstractBackgroundLimonene and linalool are used in cosmetic products for their floral scents, but their oxidation products are strong contact allergens whose mechanisms of action are still not fully understood.ObjectivesThe effects of limonene hydroperoxide (Lim‐2‐OOH) and linalool hydroperoxides (Lin‐6/7‐OOH) on the lipid profile of a human keratinocyte cell line (HaCaT) were evaluated. 2,4‐Dinitrofluorobenzene (DNFB) was also included.MethodsLim‐2‐OOH and Lin‐6/7‐OOH were synthesised according to previous methods. HaCaT cells were treated with allergens (10 μM) for 24 h and the cellular lipid extracts were analysed by C18 liquid chromatography with tandem mass spectrometry (LC–MS/MS). Data analysis was performed using Lipostar software. Statistical analysis was carried out using Metaboanalyst and R software.ResultsAll three sensitisers used caused significant changes in the lipidome of HaCaT cells in a similar trend. There was an upregulation in several plasmanyl/plasmenyl phospholipids (O‐/P‐phosphatidylcholines [PC] and O‐/P‐phosphatidylethanolamines [PE]), sphingolipids (HexCer) and triacylglycerol lipid species, and a decrease in some polyunsaturated fatty acids‐containing phospholipid (PE and PC) species suggesting oxidative stress and inflammation.ConclusionsThis study is the first to evaluate the plasticity of the HaCaT cell lipidome in response to allylic hydroperoxide allergens Lim‐2‐OOH and Lin‐6/7‐OOH, together with the experimental contact allergen DNFB. These allergens are able to upregulate and downregulate certain lipid classes to a varying degree. 10.1111/cod.14711 http://creativecommons.org/licenses/by-nc/4.0/ |
| title | A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes |
| topic | Contact Dermatitis |
| url | https://onlinelibrary.wiley.com/doi/10.1111/cod.14711 |