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Main Authors: Charlotte Jasmin Kiani, Valentina Faihs, Claudia Kugler, Neslim Ercan, Shyami Kandage, Susanne Kaesler, Tilo Biedermann, Knut Brockow
Format: Artículo Open Access
Published: Wiley 2026
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Online Access:https://onlinelibrary.wiley.com/doi/10.1111/cod.70080
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author Charlotte Jasmin Kiani
Valentina Faihs
Claudia Kugler
Neslim Ercan
Shyami Kandage
Susanne Kaesler
Tilo Biedermann
Knut Brockow
author_facet Charlotte Jasmin Kiani
Valentina Faihs
Claudia Kugler
Neslim Ercan
Shyami Kandage
Susanne Kaesler
Tilo Biedermann
Knut Brockow
Charlotte Jasmin Kiani
Valentina Faihs
Claudia Kugler
Neslim Ercan
Shyami Kandage
Susanne Kaesler
Tilo Biedermann
Knut Brockow
collection Wiley Open Access
contents Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance Spectroscopy and Corneometry Charlotte Jasmin Kiani Valentina Faihs Claudia Kugler Neslim Ercan Shyami Kandage Susanne Kaesler Tilo Biedermann Knut Brockow Contact Dermatitis ABSTRACT Background Skin barrier disruption can be induced through experimental models, yet standardised comparisons are scarce. Transepidermal water loss (TEWL) and corneometry (CM) are established methods to quantify barrier impairment. Electrical impedance spectroscopy (EIS) is a novel approach, which may provide complementary information. Objectives To compare EIS against TEWL and CM and to assess different barrier disruption models in humans in vivo. Methods 15 healthy adults (7 female, 8 male; median age 27 years) were recruited. Experiment I compared 3 adhesive tapes for tape stripping (TS). Experiment II evaluated TS, 0.5% sodium lauryl sulphate (SLS), SLS + TS, and gluten using TEWL, EIS, and CM. Results D‐Squame tape caused stronger barrier impairment than Scotch and Tesa (TEWL p  < 0.01, EIS p  < 0.05). SLS + TS induced the most (8 h: TEWL p  < 0.001; EIS p  < 0.05; CM p  < 0.05) and persistent disruption (24 h: TEWL p  < 0.05, EIS p  < 0.05), followed by TS (8 h: TEWL p  < 0.01; EIS p  < 0.05). SLS and aqua caused minor, non‐significant effects; gluten had no measurable impact. EIS correlated strongly with TEWL ( ρ  = −0.62, p  < 0.0001) and CM ( ρ  = −0.61, p  < 0.0001). Conclusions This first human study of EIS after experimental barrier disruption demonstrated strong concordance with TEWL and CM. Combined mechanical and chemical stress best reflects real‐life barrier insults and represents a potent model. 10.1111/cod.70080 http://creativecommons.org/licenses/by/4.0/
doi_str_mv 10.1111/cod.70080
format Artículo Open Access
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institution Wiley Open Access
license_str_mv http://creativecommons.org/licenses/by/4.0/
publishDate 2026
publisher Wiley
record_format wiley_oa
spellingShingle Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance Spectroscopy and Corneometry
Charlotte Jasmin Kiani
Valentina Faihs
Claudia Kugler
Neslim Ercan
Shyami Kandage
Susanne Kaesler
Tilo Biedermann
Knut Brockow
Contact Dermatitis
Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance Spectroscopy and Corneometry Charlotte Jasmin Kiani Valentina Faihs Claudia Kugler Neslim Ercan Shyami Kandage Susanne Kaesler Tilo Biedermann Knut Brockow Contact Dermatitis ABSTRACT Background Skin barrier disruption can be induced through experimental models, yet standardised comparisons are scarce. Transepidermal water loss (TEWL) and corneometry (CM) are established methods to quantify barrier impairment. Electrical impedance spectroscopy (EIS) is a novel approach, which may provide complementary information. Objectives To compare EIS against TEWL and CM and to assess different barrier disruption models in humans in vivo. Methods 15 healthy adults (7 female, 8 male; median age 27 years) were recruited. Experiment I compared 3 adhesive tapes for tape stripping (TS). Experiment II evaluated TS, 0.5% sodium lauryl sulphate (SLS), SLS + TS, and gluten using TEWL, EIS, and CM. Results D‐Squame tape caused stronger barrier impairment than Scotch and Tesa (TEWL p  < 0.01, EIS p  < 0.05). SLS + TS induced the most (8 h: TEWL p  < 0.001; EIS p  < 0.05; CM p  < 0.05) and persistent disruption (24 h: TEWL p  < 0.05, EIS p  < 0.05), followed by TS (8 h: TEWL p  < 0.01; EIS p  < 0.05). SLS and aqua caused minor, non‐significant effects; gluten had no measurable impact. EIS correlated strongly with TEWL ( ρ  = −0.62, p  < 0.0001) and CM ( ρ  = −0.61, p  < 0.0001). Conclusions This first human study of EIS after experimental barrier disruption demonstrated strong concordance with TEWL and CM. Combined mechanical and chemical stress best reflects real‐life barrier insults and represents a potent model. 10.1111/cod.70080 http://creativecommons.org/licenses/by/4.0/
title Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance Spectroscopy and Corneometry
topic Contact Dermatitis
url https://onlinelibrary.wiley.com/doi/10.1111/cod.70080