Development of a Generic Analysis Method for Isothiazolinones in Consumer Products Prompted by Increased Sensitisation to Benzisothiazolinone

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Autori principali: Anne Farbrot, Jakob Kentson, Hatice Koca Akdeve, Anders Blom, Britt‐Marie Ehn, Naida Babic Mulic, Firoozeh Amirbeagi, Mikael Alsterholm, Lina Hagvall
Natura: Artículo Open Access
Pubblicazione: Wiley 2026
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author Anne Farbrot
Jakob Kentson
Hatice Koca Akdeve
Anders Blom
Britt‐Marie Ehn
Naida Babic Mulic
Firoozeh Amirbeagi
Mikael Alsterholm
Lina Hagvall
author_facet Anne Farbrot
Jakob Kentson
Hatice Koca Akdeve
Anders Blom
Britt‐Marie Ehn
Naida Babic Mulic
Firoozeh Amirbeagi
Mikael Alsterholm
Lina Hagvall
Anne Farbrot
Jakob Kentson
Hatice Koca Akdeve
Anders Blom
Britt‐Marie Ehn
Naida Babic Mulic
Firoozeh Amirbeagi
Mikael Alsterholm
Lina Hagvall
collection Wiley Open Access
contents Development of a Generic Analysis Method for Isothiazolinones in Consumer Products Prompted by Increased Sensitisation to Benzisothiazolinone Anne Farbrot Jakob Kentson Hatice Koca Akdeve Anders Blom Britt‐Marie Ehn Naida Babic Mulic Firoozeh Amirbeagi Mikael Alsterholm Lina Hagvall Contact Dermatitis ABSTRACT Background Isothiazolinones are widely used as preservatives in both cosmetic and non‐cosmetic products. Sensitisation to isothiazolinones other than chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT), present in the baseline series, is increasing. Objectives To investigate the prevalence of sensitisation to benzisothiazolinone (BIT) in comparison to CMIT/MIT and to develop a product‐independent analysis method of isothiazolinones for laboratories with basic instrumentation. Patients and Methods Patch testing with BIT and CMIT/MIT was performed in 803 consecutively tested dermatitis patients. A simple and general analysis method for isothiazolinones was developed for HPLC‐UV. Results Investigation of 14 products labelled to contain BIT and/or CMIT/MIT (cosmetic and non‐cosmetic) showed concentrations of BIT in non‐cosmetic products of up to 272 ppm. Maximum detected concentrations of CMIT and MIT were 0.3 and 1.5 ppm, respectively. Dichloroctylisothiazolinone and MIT were detected in an unlabelled hobby paint. BIT was a significantly more common cause of sensitisation than CMIT/MIT, at 7.7% and 5.2%, respectively. Conclusions Results confirm previous reports indicating that sensitisation to BIT is increasing in dermatitis patients in western Sweden. Analysis of products showed that concentrations of BIT can be several orders of magnitude higher than those of CMIT/MIT in products to which skin contact can be frequent and long lasting. 10.1111/cod.70147 http://creativecommons.org/licenses/by/4.0/
doi_str_mv 10.1111/cod.70147
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spellingShingle Development of a Generic Analysis Method for Isothiazolinones in Consumer Products Prompted by Increased Sensitisation to Benzisothiazolinone
Anne Farbrot
Jakob Kentson
Hatice Koca Akdeve
Anders Blom
Britt‐Marie Ehn
Naida Babic Mulic
Firoozeh Amirbeagi
Mikael Alsterholm
Lina Hagvall
Contact Dermatitis
Development of a Generic Analysis Method for Isothiazolinones in Consumer Products Prompted by Increased Sensitisation to Benzisothiazolinone Anne Farbrot Jakob Kentson Hatice Koca Akdeve Anders Blom Britt‐Marie Ehn Naida Babic Mulic Firoozeh Amirbeagi Mikael Alsterholm Lina Hagvall Contact Dermatitis ABSTRACT Background Isothiazolinones are widely used as preservatives in both cosmetic and non‐cosmetic products. Sensitisation to isothiazolinones other than chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT), present in the baseline series, is increasing. Objectives To investigate the prevalence of sensitisation to benzisothiazolinone (BIT) in comparison to CMIT/MIT and to develop a product‐independent analysis method of isothiazolinones for laboratories with basic instrumentation. Patients and Methods Patch testing with BIT and CMIT/MIT was performed in 803 consecutively tested dermatitis patients. A simple and general analysis method for isothiazolinones was developed for HPLC‐UV. Results Investigation of 14 products labelled to contain BIT and/or CMIT/MIT (cosmetic and non‐cosmetic) showed concentrations of BIT in non‐cosmetic products of up to 272 ppm. Maximum detected concentrations of CMIT and MIT were 0.3 and 1.5 ppm, respectively. Dichloroctylisothiazolinone and MIT were detected in an unlabelled hobby paint. BIT was a significantly more common cause of sensitisation than CMIT/MIT, at 7.7% and 5.2%, respectively. Conclusions Results confirm previous reports indicating that sensitisation to BIT is increasing in dermatitis patients in western Sweden. Analysis of products showed that concentrations of BIT can be several orders of magnitude higher than those of CMIT/MIT in products to which skin contact can be frequent and long lasting. 10.1111/cod.70147 http://creativecommons.org/licenses/by/4.0/
title Development of a Generic Analysis Method for Isothiazolinones in Consumer Products Prompted by Increased Sensitisation to Benzisothiazolinone
topic Contact Dermatitis
url https://onlinelibrary.wiley.com/doi/10.1111/cod.70147