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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo Open Access |
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Wiley
2024
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| Accès en ligne: | https://onlinelibrary.wiley.com/doi/10.1111/exd.15021 |
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| author | Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita |
| author_facet | Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita |
| collection | Wiley Open Access |
| contents | Integrin α5 regulates motility of human monocyte‐derived Langerhans cells during immune response Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita Experimental Dermatology AbstractLangerhans cells (LCs) are mainly present in the epidermis and mucosa, and have important roles during skin infection. Migration of LCs to lymph nodes is essential for antigen presentation. However, due to the difficulties in isolating and culturing human LCs, it is not fully understood how LCs move and interact with the extracellular matrix (ECM) through their adhesion molecules such as integrin, during the immune responses. In this study, we aimed to investigate LC motility, cell shape and the role of integrin under inflammatory conditions using monocyte‐derived Langerhans cells (moLCs) as a model. As a result, lipopolysaccharide (LPS) stimulation increased adhesion on fibronectin coated substrate and integrin α5 expression in moLCs. Time‐lapse imaging of moLCs revealed that stimulation with LPS elongated cell shape, whilst decreasing their motility. Additionally, this decrease in motility was not observed when pre‐treated with a neutralising antibody targeting integrin α5. Together, our data suggested that activation of LCs decreases their motility by promoting integrin α5 expression to enhance their affinity to the fibronectin, which may contribute to their migration during inflammation. 10.1111/exd.15021 http://creativecommons.org/licenses/by/4.0/ |
| doi_str_mv | 10.1111/exd.15021 |
| format | Artículo Open Access |
| id | wiley_oa_10_1111_exd_15021 |
| institution | Wiley Open Access |
| license_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | wiley_oa |
| spellingShingle | Integrin α5 regulates motility of human monocyte‐derived Langerhans cells during immune response Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita Experimental Dermatology Integrin α5 regulates motility of human monocyte‐derived Langerhans cells during immune response Zhihan Guo Masato Murakami Kaori Saito Hiroko Kato Manami Toriyama Makoto Tominaga Ken J. Ishii Fumitaka Fujita Experimental Dermatology AbstractLangerhans cells (LCs) are mainly present in the epidermis and mucosa, and have important roles during skin infection. Migration of LCs to lymph nodes is essential for antigen presentation. However, due to the difficulties in isolating and culturing human LCs, it is not fully understood how LCs move and interact with the extracellular matrix (ECM) through their adhesion molecules such as integrin, during the immune responses. In this study, we aimed to investigate LC motility, cell shape and the role of integrin under inflammatory conditions using monocyte‐derived Langerhans cells (moLCs) as a model. As a result, lipopolysaccharide (LPS) stimulation increased adhesion on fibronectin coated substrate and integrin α5 expression in moLCs. Time‐lapse imaging of moLCs revealed that stimulation with LPS elongated cell shape, whilst decreasing their motility. Additionally, this decrease in motility was not observed when pre‐treated with a neutralising antibody targeting integrin α5. Together, our data suggested that activation of LCs decreases their motility by promoting integrin α5 expression to enhance their affinity to the fibronectin, which may contribute to their migration during inflammation. 10.1111/exd.15021 http://creativecommons.org/licenses/by/4.0/ |
| title | Integrin α5 regulates motility of human monocyte‐derived Langerhans cells during immune response |
| topic | Experimental Dermatology |
| url | https://onlinelibrary.wiley.com/doi/10.1111/exd.15021 |